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Application of vanilline in preparation of osteoclast differentiation inhibitor

A technology of vanillinine and osteoclasts, applied in the field of biomedicine, can solve problems such as mandibular osteonecrosis, venous thrombosis, and the inability to significantly reduce the risk of atypical fractures

Active Publication Date: 2020-08-07
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these drugs can improve the bone mineral density of patients with osteoporosis to a certain extent, they cannot significantly reduce the risk of atypical fractures, and there are various side effects at the same time, such as bisphosphonates can cause osteonecrosis of the jaw, raloxix Fen can cause venous thrombosis

Method used

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  • Application of vanilline in preparation of osteoclast differentiation inhibitor
  • Application of vanilline in preparation of osteoclast differentiation inhibitor
  • Application of vanilline in preparation of osteoclast differentiation inhibitor

Examples

Experimental program
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Embodiment 1

[0017] Example 1: Osteoclast Differentiation Inhibitory Activity Test

[0018]

[0019] 1. Experimental method

[0020] Cell culture: take 8-week-old C57BL / 6 mouse femoral marrow cells, and use MEM-α complete medium containing 10ng / mL M-CSF (containing 10% fetal bovine serum (FBS), 100U / mL penicillin and 100μg / mL streptomycin), cultured in a carbon dioxide incubator at 37°C for 24 hours, discarded the culture medium, washed the cells with phosphate buffered saline (PBS) slowly for 1 or 2 times, removed the suspended cells, and then washed them with 4mL MEM - α complete medium Gently blow off the adherent cells and centrifuge at 1000RPM / min for 10min to obtain mouse bone marrow macrophages (BMMs).

[0021] Induce osteoclast formation: BMMs cells were diluted to a density of 1×10 with MEM-α complete medium containing 10 ng / mL LM-CSF cytokine 5 cells / mL of cell suspension, and seeded in 96-well plate, 100 μL per well. After culturing for 24 hours to adhere to the wall, the...

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Abstract

The invention discloses an application of vanilline in preparation of an osteoclast differentiation inhibitor. According to the application of the vanilline in preparation of the osteoclast differentiation inhibitor, wherein the structural formula of the vanilline is shown as a formula 1. It is found through tests that vanilline has a direct and remarkable inhibition effect on osteoclast differentiation, and therefore vanilline can serve as an osteoclast differentiation inhibitor medicine to be used for treating various diseases such as osteoporosis caused by osteoclast differentiation.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of vanillinine in the preparation of osteoclast differentiation inhibitors. Background technique [0002] Osteoporosis is a systemic bone metabolic disease. With the prolongation of human life span and the acceleration of population aging, the incidence of osteoporosis is increasing year by year. It has become one of the important chronic diseases that affect people's quality of life. public health issues. According to statistics at the 2014 American Society for Bone and Mineral Research (ASBMR) annual meeting, osteoporotic fractures are one of the most important chronic diseases in the 21st century, and 1 / 2 of the elderly women and 1 / 3 of the elderly men in the world will suffer from osteoporosis. Loose fractures. Therefore, the development of effective anti-osteoporosis drugs is of great significance for improving the quality of life of humans, especiall...

Claims

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Application Information

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IPC IPC(8): A61K31/4355A61P19/10
CPCA61K31/4355A61P19/10
Inventor 刘志红黄丹娥徐峻谢黎炜顾琼郑双佳许国焕韩木兰
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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