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Application of CD317 protein in prevention and treatment of virus infection

A virus infection, protein technology, used in antiviral agents, peptide/protein components, medical preparations containing active ingredients, etc.

Active Publication Date: 2020-08-11
JIANGSU PURECELL BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although CD317 can inhibit the exit of various enveloped viruses through its special topological structure, it can inhibit human immunodeficiency virus type 1 (Human immunodeficiency virus 1, HIV-1), influenza virus type A (FluA), Ebola virus (Ebola virus virus), human immunodeficiency virus type 2 (Human immunodeficiency virus 2, HIV-2), Japanese encephalitis virus (Japanese encephalitis virus), Sendai virus (Sendai virus), human herpes virus α subtype herpes simplex virus type 1 ( Herpes simplex virus 1, HSV-1) and herpes simplex virus 2 (Herpessimple virus 2, HSV-2), Kaposi's Sarcoma-Associated Herpesvirus (KSHV) in the human herpesvirus gamma subtype , coronavirus (coronavirus229E) and other enveloped viruses have the effect of inhibiting infection, but existing studies have shown that CD317 does not have the effect of inhibiting infection for all enveloped viruses

Method used

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  • Application of CD317 protein in prevention and treatment of virus infection
  • Application of CD317 protein in prevention and treatment of virus infection
  • Application of CD317 protein in prevention and treatment of virus infection

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Experimental program
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Effect test

Embodiment 1

[0027] 1. Construction of T cells with up-regulated expression of CD317

[0028] Among them, lentiviral packaging plasmids such as CS-CA-MCS, pCAG-HIV-gp, and pCMV-VSV-G-RSV-Rev were obtained through MTA (Material Transfer Agreements, MTA).

[0029]I. Obtain the human CD317 gene sequence through NCBI query, design and synthesize primers.

[0030] The human CD317 gene sequence (NCBI accession number: NM_004335.4) is 540 bases in length, encoded by 180 amino acids, and the relevant primers are as follows:

[0031] CD317xho-F: 5'-ACACTCGAGCCACCATGGCATCTACTTCGTA-3'

[0032] CD317435-R: 5'-CTTGTCGTCATCGTCTTTGTAGTCGCTTAAGACCTGGTTTTC-3'

[0033] CD317436Flag-F: 5'-ACAAAGACGATGACGACAAGGTGAGAATCGCGGACAAG-3'

[0034] CD317xho-R: 5'-ACACTCGAGTCACTGCAGCAGAGCG-3'

[0035] CSCA4459-R: 5'-AGCAGCGTATCCACATAGCG-3'

[0036] U31-F: 5'-TTTGCAGTCATCACGATCGG-3'

[0037] U31-R: 5'-AGAGCGACAAATTGGAGGTTTC-3'

[0038] II. Construction of CSCA-CD317Flag plasmid

[0039] 1. Extraction of total ...

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Abstract

The invention discloses an application of CD317 in prevention and / or treatment of human herpes virus 6 (HHV-6) infection. The application comprises the following steps: constructing an expression vector for expressing a human CD317 gene, further constructing host cells of which CD317 expression is up-regulated, infecting the host cells of which the CD317 expression is up-regulated by using an HHV-6 virus strain, and then detecting the expression level of the early protein IE1 and the change of the virus productivity. Compared with a Control group, the CD317 expression up-regulation can significantly reduce the expression of the early protein IE1 and reduce the total yield of the virus; therefore, the CD317 protein can significantly inhibit HHV-6 virus proliferation and reduce HHV-6 virus invasion and infection on host cells, has significant anti-HHV-6 virus activity, and can be used for preparing drugs for preventing and / or treating HHV-6 infection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of CD317 protein in the prevention and treatment of virus infection, in particular to the application of CD317 protein in the preparation of medicaments for the prevention and / or treatment of HHV-6 infection. Background technique [0002] Human herpesvirus 6 (human herpesvirus 6, HHV-6) belongs to the subtype of human herpesvirus β, which is a kind of human T lymphocyte-tropic double-stranded DNA virus. Infection in the crowd is very common, and the positive rate of HHV-6 antibody in the Asian population is more than 90%. HHV-6 primary infection generally occurs in infants and young children aged 6 months to 2 years, and the peak period is 6-9 months, and then establishes long-term latent infection in brain tissue, monocytes or macrophages, etc. Pathogens that cause acute eruptions in infants and young children. HHV-6 will be reactivated or re-infected when the body's...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K45/06A61P31/22
CPCA61K38/177A61K45/06A61P31/22
Inventor 孙振华徐献毅
Owner JIANGSU PURECELL BIOMEDICAL TECH CO LTD