Microorganism strengthening method for enriching cadmium by hyperaccumulator and composite suspension used by microorganism strengthening method
A composite, biomass charcoal technology, applied in chemical instruments and methods, restoration and application of polluted soil, etc., can solve problems such as plant physiological damage, and achieve the effects of low cost, simple method and reducing soil cadmium content.
Active Publication Date: 2020-08-11
ZHEJIANG UNIV
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Problems solved by technology
But at the same time, under heavy metal stress, the time required for phytoremediation and the physiological damage to plants under high pollution conditions are the main problems faced by phytoremediation.
Method used
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Embodiment 1
[0030] Embodiment 1, a kind of preparation method of bacterial strain-carbon-based saline complex suspension, carries out following steps successively:
[0031] 1), prepare the thalline of cadmium-resistant bacteria M6:
[0032] Inoculate the cadmium-resistant bacteria M6 into the liquid LB medium, shake the bacteria to the logarithmic phase at a temperature of 28°C and a speed of 200r / min under aerobic conditions, and adjust the OD value with a UV spectrophotometer according to the growth curve of the bacteria , to ensure that the bacterial liquid cfu ≥ 1 × 10 8 cells / mL, centrifuge at 8000r / min for 10min, discard the supernatant, and obtain the bacterial cells for use.
[0033] 2) Collect pine and bamboo from the wild, first place them in an oven at 60°C for drying and dehydration for 20 hours, then dry them in an oven at 48°C for 28 hours, take them out, crush them with a crusher, and pass through a 1mm sieve to obtain pine and bamboo particles;
[0034] The pine and bamb...
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The invention discloses a preparation method of a strain-carbon-based saline complex suspension. The preparation method comprises the following steps: preparing thalli of cadmium-resistant bacteria M6; preparing biomass charcoal from pine bamboos; adding the pine bamboo biomass charcoal into normal saline, and adjusting the pH value to be neutral; and adding the thalli into the obtained carbon-based normal saline, and performing culturing to obtain a strain-carbon-based saline complex suspension. The invention further provides a microorganism strengthening method for enriching cadmium by a hyperaccumulator. The microorganism strengthening method comprises the following steps: planting sedum plumbizincicola in cadmium-contaminated soil, and applying the strain-carbon-based saline complex suspension once every 8-12 days. According to the microorganism strengthening method, on the basis of increasing the field activity of a cadmium-tolerant microbial inoculum, the cadmium enrichment capacity of the hyperaccumulator is improved, and the method is simple, convenient, low in cost and wide in application prospect.
Description
technical field [0001] The invention relates to a microbial strengthening method for superaccumulating phytoremediation of soil cadmium pollution, and the used bacterial strain-carbon-based salt water complex suspension. Background technique [0002] With the continuous advancement of my country's industrialization process, the problem of heavy metal pollution in soil has become increasingly serious and has attracted more and more attention. At the same time, people's research on pollution control methods has become more and more in-depth. Phytoremediation has attracted widespread attention because of its low price, simple operation, less environmental disturbance, and more thorough treatment effect. But at the same time, under heavy metal stress, the time required for phytoremediation and the physiological damage to plants under high pollution conditions are the main problems faced by phytoremediation. Therefore, with the deepening of research, it has been found that the i...
Claims
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Login to View More IPC IPC(8): C09K17/40B09C1/10B09C1/08C12N11/14
CPCB09C1/08B09C1/10B09C1/105B09C2101/00C09K17/40C12N11/14
Inventor 张奇春王京文王浣荷金树权
Owner ZHEJIANG UNIV