High-temperature strain compounding method for treating feces

A technology of high-temperature bacteria and manure, which is applied in the direction of biological sludge treatment and separation of microorganisms, can solve the problems of medium formula limitation, inability to replicate, and low decomposition efficiency, so as to shorten the composting cycle, promote rapid decomposition, and improve Effect of degree of maturity and degree of stabilization

Pending Publication Date: 2020-08-11
南京威马农业有限公司
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  • Summary
  • Abstract
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Problems solved by technology

[0003] At present, the high-temperature strains of ectopic fermentation beds used in domestic treatment of feces mainly rely on imports from Japan, and the price is about 120-150 yuan / kg. Imported products are strictly limited by the medium formula when they are used for expansion.
Japan masters the core strain compounding plan and does not publish it. The medium plan for expansion is too complicated and cannot be replicated for domestic use. In addition, the existing degrading strains are difficult to adapt to high temperature, high humidity and carbon-nitrogen ratio imbalance environment, and decompose less efficient

Method used

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Examples

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Effect test

Embodiment 1

[0023] A method for compounding high-temperature strains for treating manure, comprising the following steps:

[0024] Step 1: Preliminary screening. After the sample is made into a suspension and enriched at 70°C at high temperature, the sample suspension is made into 60%, 80% and 100% dilution of the bacterial solution and spread on the beef extract peptone plate, inverted and cultured in a 70°C incubator 24h. The colonies with different morphological characteristics were picked out, streaked and purified on the beef extract peptone plate for 5 times, and high temperature resistant pure strains were isolated. Inoculate the isolated high-temperature-resistant pure strains into cellulose Congo red medium, starch selective medium, and protein selective medium, and observe whether there is a transparent hydrolysis circle around the colony. If a transparent hydrolysis circle appears, it means that the substrate has been hydrolyzed, and the strain has the ability to degrade the ...

Embodiment 2

[0034] A method for compounding high-temperature strains for treating manure, comprising the following steps:

[0035] Step 1: Preliminary screening. After the sample suspension was enriched at a high temperature of 75°C, the sample suspension was made into 60%, 80% and 100% dilution bacterial solutions and spread on the beef extract peptone plate, inverted and placed in a 75°C incubator Cultivate for 24h. The colonies with different morphological characteristics were picked out, streaked and purified on the beef extract peptone plate for 5 times, and high temperature resistant pure strains were isolated. Inoculate the isolated high-temperature-resistant pure strains into cellulose Congo red medium, starch selective medium, and protein selective medium, and observe whether there is a transparent hydrolysis circle around the colony. If a transparent hydrolysis circle appears, it means that the substrate has been hydrolyzed, and the strain has the ability to degrade the substr...

Embodiment 3

[0045] A method for compounding high-temperature strains for treating manure, comprising the following steps:

[0046] Step 1: Preliminary screening. After the sample is made into a suspension and enriched at 80°C at high temperature, the sample suspension is made into 60%, 80% and 100% dilution of the bacterial solution and spread on the beef extract peptone plate, inverted and cultured in an 80°C incubator 24h. The colonies with different morphological characteristics were picked out, streaked and purified on the beef extract peptone plate for 5 times, and high temperature resistant pure strains were isolated. Inoculate the isolated high-temperature-resistant pure strains into cellulose Congo red medium, starch selective medium, and protein selective medium, and observe whether there is a transparent hydrolysis circle around the colony. If a transparent hydrolysis circle appears, it means that the substrate has been hydrolyzed, and the strain has the ability to degrade the...

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Abstract

The invention discloses a high-temperature strain compounding method for treating feces. The high-temperature strain compounding method for treating feces comprises the following steps of preparing asample into a suspension, performing enriching at high temperature of 70-80 DEG C, preparing the sample suspension into 60%, 80% and 100% diluted bacterial solutions, coating a beef extract peptone plate with the bacterial solutions separately, inverting the beef extract peptone plate, and culturing in a 70-80 DEG C incubator for 24 hours; and selecting bacterial colonies with different morphological characteristics, and carrying out streaking purification and separation on the beef extract peptone flat plate for 5 times to obtain a high-temperature-resistant pure strain. According to the method, the high-temperature bacteria with the capability of simultaneously degrading three organic macromolecules, namely cellulose, protein and starch, are screened from a humus sample in an ectopic fermentation bed feces accumulation fermentation process, excellent strains are provided for composting, rapid decomposition of composting materials is effectively promoted, and the composting period isshortened.

Description

technical field [0001] The invention relates to the technical field of high-temperature bacterial strains in ectopic fermentation beds, in particular to a method for compounding high-temperature bacterial strains for treating manure. Background technique [0002] High-temperature bacteria have the characteristics of fast metabolism, high activity, short generation time, high thermal stability of enzymes, and the creation of high-temperature conditions to kill pathogenic bacteria. They have an immeasurable effect on the biotransformation of organic substances in high-temperature environments. Higher microbial metabolic activity and organic matter degradation rate have broad application prospects in the field of solid waste utilization. [0003] At present, the high-temperature strains of ectopic fermentation beds used in domestic treatment of feces mainly rely on imports from Japan, and the price is 120-150 yuan / kg. Japan masters the core strain compounding plan and does not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C02F11/04
CPCC12N1/02C02F11/04
Inventor 徐杨潘孝青张磊
Owner 南京威马农业有限公司
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