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Application of Serratia marcescens in the preparation of termite repellent

A technology of Serratia marcescens and repellent, which is applied in the directions of animal repellent, application, and pest repellent, and can solve the problems of human body and ancient wood damage, ecological environment damage, and great harm.

Active Publication Date: 2021-05-18
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of prevention and control is still chemical prevention and control, which is very harmful and seriously damages the ecological environment. Especially in urban prevention and control and protection of ancient trees, it is difficult to use general methods to achieve good results, and it may cause harm to the human body and ancient trees. a certain degree of harm

Method used

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  • Application of Serratia marcescens in the preparation of termite repellent
  • Application of Serratia marcescens in the preparation of termite repellent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Serratia marcescens used in this experiment was isolated from dead termites in the laboratory, and was preserved in Guangdong Microbial Culture Collection Center (GDMCC) on April 17, 2020, address: Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou On the 5th floor, the preservation number is: GDMCC No: 60999.

[0016] Serratia marcescens was inoculated in LB liquid medium, and cultured on a shaker at 200 r / min for 48 hours at 25±1°C to obtain Serratia marcescens culture fluid (bacteria liquid) for use.

[0017] The soil is retrieved from the environment where termites exist in the wild, dried and crushed for later use.

[0018] 50mL of bacterial liquid was applied to 500g of soil as a treatment for Serratia marcescens, and 50mL of LB culture solution was applied to 500g of soil as a control treatment without Serratia marcescens. 25g of Serratia marcescens soil and 25g of Serratia marcescens soil were placed on both sides of a 9cm diameter petri dish respect...

Embodiment 2

[0024] The soil is retrieved from the environment where termites exist in the field, dried and crushed for later use.

[0025] Serratia marcescens was isolated from dead termites in the laboratory, inoculated in LB liquid medium, and cultured on a 200r / min shaker for 48 hours at 25±1°C to obtain Serratia marcescens culture solution ( Bacterial solution), ready to use.

[0026] 50mL of bacterial liquid was applied to 500g of soil as a treatment for Serratia marcescens, and 50mL of LB culture solution was applied to 500g of soil as a control treatment without Serratia marcescens. 30g of Serratia marcescens soil and 30g of Serratia marcescens soil were placed on both sides of a 9cm diameter petri dish respectively, with a plastic strip in the middle. Place this treatment in a dry and ventilated environment for 10 days before use.

[0027] Spray pure water to make the soil moist, and place a piece of wet filter paper in the center of the soil on both sides for the termites to fe...

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Abstract

The invention discloses the application of Serratia marcescens in preparing termite repellent. The Serratia marcescens is a termite repellent prepared in the form of a Serratia marcescens strain culture solution. The present invention verifies that the Serratia marcescens strain culture solution can be used as a termite by mixing and applying the Serratia marcescens strain culture solution to the soil, after releasing the termites, and observing the repelling situation of the termites. The repellent is applied, and its repellent effect is persistent. And because Serratia marcescens is simple to cultivate, has a short growth period, a fast growth rate, and is easy to expand and cultivate, it is not a chemical agent, and has good conditions for application as a termite repellent.

Description

technical field [0001] The invention relates to the technical field of termite control, in particular to the application of Serratia marcescens in the preparation of termite repellents. Background technique [0002] Termites are a worldwide important pest, especially in tropical and subtropical regions. Our country has been found to be distributed in 23 provinces and cities, and the endangered area is close to 40% of the total area of ​​the country. They are highly proliferative and quick to destroy, causing huge damage in a short period of time. The economic loss caused by termite damage is about 2 billion to 2.5 billion yuan per year (Li Xiaoying and Wang Yiyan, 2003). At present, the main method of prevention and control is still chemical prevention and control, which is very harmful and seriously damages the ecological environment. Especially in urban prevention and control and protection of ancient trees, it is difficult to use general methods to achieve good results,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/20A01P17/00C12N1/20C12R1/43
CPCA01N63/20C12N1/205C12R2001/43
Inventor 陈勇曾文慧李志强刘炳荣吴文静
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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