An indel marker primer pair and method for identifying the purity of Cucumber Lvmei No. 1 hybrid seeds

Abstract

The invention discloses a pair of Indel marker primers for the purity identification of Cucumber Lvmei No. 1 hybrid seeds. The markers include an Indel 79 upstream primer Indel 79-F and a downstream primer Indel 79-R, and the upstream primer Indel 79-R The nucleotide sequence of F is shown in SEQ ID NO.1, and the nucleotide sequence of the downstream primer Indel 79‑R is shown in SEQ ID NO.2. The invention also discloses a method for identifying the purity of cucumber Lvmei No. 1 hybrid seeds. With the primers and method of the invention, it is only necessary to simply extract the genomic DNA of cucumber leaves, and then carry out PCR amplification and agarose gel electrophoresis. The effective identification of the purity of Lvmei No. 1 cucumber hybrid seeds can replace the field purity identification method, greatly shorten the identification cycle, save identification costs, improve identification accuracy, and have strong commercial application value.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to an Indel marker primer pair and a method for identifying the purity of Cucumber Lvmei No. 1 hybrid seeds. Background technique [0002] Cucumber is one of the vegetables with the largest planting area in my country, and it is also one of the main economic crops in China. At present, with the gradual expansion of the cultivation area of ​​protected areas and glass greenhouses, the cultivation of cucumbers has also changed from one crop a year to three crops a year. Cucumber varieties are also gradually enriched, and they no longer exist as a single vegetable. Since different regions have different demands for cucumber varieties, breeders have to select and breed different types of cucumbers according to regions. At the same time, seed quality is also quite important, and seed purity is one of the important indicators to measure seed quality. [0003] At pr...

AI Technical Summary

Problems solved by technology

In the process of cucumber hybrid breeding, due to reasons such as pollen mixing and insect pollination, false hybrids often appear, resulting in a decrease in seed purity and seed quality, causing huge economic losses in production and sales

The traditional purity identification is mainly morphological identification through field regional tests. Although the identification results are relatively accurate, the determination of plant morphology takes a long time, which is difficult to meet the needs of rapid determination during the investigation process. Big

Images