Bacillus polymyxa water-soluble fertilizer, and preparation method and application thereof
A technology of Bacillus polymyxa and water-soluble fertilizers, applied in the field of biological fertilizers, can solve problems such as increasing the cost of pest control, pathogenic bacteria producing drug resistance, and reducing the commodity value of fruits, so as to improve disease resistance, crop quality, and photosynthesis. exuberant effect
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Embodiment 1
[0024] The screening of embodiment 1 bacillus polymyxa LTPP03
[0025] 1) Sample collection: collect soil (forest land in Sitang Town, Xingning District, Nanning City), and place it in a container;
[0026] 2) Dilution and heating in a water bath: Take 10g of soil and put it into a 250ml beaker, add 90ml of sterile water into the beaker, oscillate to make a concentration of 10 -1 Soil dilution A; place soil dilution A in a water bath at 80°C and heat for 10 minutes;
[0027] 3) Gradient dilution:
[0028] (1) Take 3 test tubes filled with 9mL sterile water, marked as: 10 -2 、10 -3 and 10 -4 ;
[0029] (2) Shake the soil dilution solution A and let it rest for 30s. Use a pipette to draw 1mL of the soil dilution solution and add the solution marked with 10 -2 In the test tube, and gently blow and suck several times in the test tube to make it fully mixed, the soil dilution B is obtained;
[0030] (3) Similarly, draw 1mL of soil dilution B and add the number 10 -3 In a te...
Embodiment 2
[0042] The identification of embodiment 2 bacillus polymyxa LTPP03
[0043] The China Center for Type Culture Collection identified the Paenibacillus polymyxa LTPP03 strain on February 28, 2019. Identification includes morphological observation of Paenibacillus polymyxa LTPP03 strain, tests of physiological and biochemical characteristics, determination and analysis of 16SrRNA gene sequence, and the taxonomic status of the strain is determined according to the identification results. The bacterial strain was identified as: Paenibacillus polymyxa LTPP03 (Paenibacillus polymyxa LTPP03). The test results of physiological and biochemical characteristics are shown in Table 1-Table 2;
[0044] Table 1 Physiological and biochemical characteristics of strain LTPP03 - enzyme activity, carbon source assimilation
[0045]
[0046]
[0047] +: Positive reaction; -: Negative reaction; w: Weak positive reaction
[0048] Table 2 Physiological and biochemical characteristics of strain ...
Embodiment 3
[0053] A preparation method of bacillus polymyxa water-soluble fertilizer, comprising the steps of:
[0054] 1) Weighing: Weigh 5 billion / gLTPP03 bacterial powder 40g, amino acid raw powder 350g, ammonium sulfate 100g, nitrogen and potassium compound fertilizer 50g, potassium sulfate 100g, diammonium hydrogen phosphate 50g, magnesium sulfate monohydrate 109g, boric acid 50g, a Zinc sulfate water 50g, manganese glycinate 5g, iron glycinate 10g, ammonium heptamolybdate 1g, copper glycinate 5g, sodium methylene bis-naphthalene sulfonate 20g, sodium lignosulfonate 40g, polyacrylamide 10g and fatty alcohol polyoxyethylene Ether 10g;
[0055] 2) Mixing: Magnesium sulfate monohydrate, boric acid, zinc sulfate monohydrate, manganese glycinate, iron glycinate, ammonium heptamolybdate, copper glycinate, LTPP03 bacterial powder, sodium methylene bis-naphthalene sulfonate and lignin Sodium sulfonate is mixed in a blender;
[0056] 3) Mixing: Stir the ammonium sulfate, nitrogen and potas...
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