Bacillus polymyxa water-soluble fertilizer, and preparation method and application thereof

A technology of Bacillus polymyxa and water-soluble fertilizers, applied in the field of biological fertilizers, can solve problems such as increasing the cost of pest control, pathogenic bacteria producing drug resistance, and reducing the commodity value of fruits, so as to improve disease resistance, crop quality, and photosynthesis. exuberant effect

Active Publication Date: 2020-08-25
广西乐土生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Citrus canker is a disease caused by Trichobacterium flavus, which mostly occurs in citrus. The disease damages citrus leaves, branches and fruits. When the seedlings and young trees are seriously damaged, it will cause fallen leaves and dead shoots, which will affect the tree vigor; the fruit will be severely damaged. The fruit will drop, and the light one will have disease scars, which will not tolerate storage and rot, which will greatly reduce the commodity value of the fruit, increase the cost of disease and pest control for the fruit grower, and damage the economic benefits.
[0003] At present, Pyrrhobacter flavus is usually prevented by spraying pesticides on citrus leaves, but on the one hand, pesticides are likely to make pathogenic bacteria resistant and reduce the difficulty of control; on the other hand, they will pollute the environment

Method used

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  • Bacillus polymyxa water-soluble fertilizer, and preparation method and application thereof
  • Bacillus polymyxa water-soluble fertilizer, and preparation method and application thereof
  • Bacillus polymyxa water-soluble fertilizer, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The screening of embodiment 1 bacillus polymyxa LTPP03

[0025] 1) Sample collection: collect soil (forest land in Sitang Town, Xingning District, Nanning City), and place it in a container;

[0026] 2) Dilution and heating in a water bath: Take 10g of soil and put it into a 250ml beaker, add 90ml of sterile water into the beaker, oscillate to make a concentration of 10 -1 Soil dilution A; place soil dilution A in a water bath at 80°C and heat for 10 minutes;

[0027] 3) Gradient dilution:

[0028] (1) Take 3 test tubes filled with 9mL sterile water, marked as: 10 -2 、10 -3 and 10 -4 ;

[0029] (2) Shake the soil dilution solution A and let it rest for 30s. Use a pipette to draw 1mL of the soil dilution solution and add the solution marked with 10 -2 In the test tube, and gently blow and suck several times in the test tube to make it fully mixed, the soil dilution B is obtained;

[0030] (3) Similarly, draw 1mL of soil dilution B and add the number 10 -3 In a te...

Embodiment 2

[0042] The identification of embodiment 2 bacillus polymyxa LTPP03

[0043] The China Center for Type Culture Collection identified the Paenibacillus polymyxa LTPP03 strain on February 28, 2019. Identification includes morphological observation of Paenibacillus polymyxa LTPP03 strain, tests of physiological and biochemical characteristics, determination and analysis of 16SrRNA gene sequence, and the taxonomic status of the strain is determined according to the identification results. The bacterial strain was identified as: Paenibacillus polymyxa LTPP03 (Paenibacillus polymyxa LTPP03). The test results of physiological and biochemical characteristics are shown in Table 1-Table 2;

[0044] Table 1 Physiological and biochemical characteristics of strain LTPP03 - enzyme activity, carbon source assimilation

[0045]

[0046]

[0047] +: Positive reaction; -: Negative reaction; w: Weak positive reaction

[0048] Table 2 Physiological and biochemical characteristics of strain ...

Embodiment 3

[0053] A preparation method of bacillus polymyxa water-soluble fertilizer, comprising the steps of:

[0054] 1) Weighing: Weigh 5 billion / gLTPP03 bacterial powder 40g, amino acid raw powder 350g, ammonium sulfate 100g, nitrogen and potassium compound fertilizer 50g, potassium sulfate 100g, diammonium hydrogen phosphate 50g, magnesium sulfate monohydrate 109g, boric acid 50g, a Zinc sulfate water 50g, manganese glycinate 5g, iron glycinate 10g, ammonium heptamolybdate 1g, copper glycinate 5g, sodium methylene bis-naphthalene sulfonate 20g, sodium lignosulfonate 40g, polyacrylamide 10g and fatty alcohol polyoxyethylene Ether 10g;

[0055] 2) Mixing: Magnesium sulfate monohydrate, boric acid, zinc sulfate monohydrate, manganese glycinate, iron glycinate, ammonium heptamolybdate, copper glycinate, LTPP03 bacterial powder, sodium methylene bis-naphthalene sulfonate and lignin Sodium sulfonate is mixed in a blender;

[0056] 3) Mixing: Stir the ammonium sulfate, nitrogen and potas...

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Abstract

The invention discloses a bacillus polymyxa water-soluble fertilizer, and a preparation method and application thereof. The water-soluble fertilizer comprises bacillus polymyxa, wherein the bacillus polymyxa is preserved in China Center for Type Culture Collection and has an accession number of CCTCC No: M2019116, and the 16srRNA sequence of the bacillus polymyxa is shown as SEQ ID No. 1. The preparation process comprises the steps of weighing, mixing and packaging. The water-soluble fertilizer can effectively prevent and treat citrus canker, reduce the morbidity of the citrus canker and effectively promote the growth of plants.

Description

technical field [0001] The present invention relates to the technical field of biological fertilizer, more specifically to a kind of bacillus polymyxa water-soluble fertilizer and its preparation method and application. Background technique [0002] Citrus canker is a disease caused by Trichobacterium flavus, which mostly occurs in citrus. The disease damages citrus leaves, branches and fruits. When the seedlings and young trees are seriously damaged, it will cause fallen leaves and dead shoots, which will affect the tree vigor; the fruit will be severely damaged. The fruit falls, and the light one has disease scars and cannot be stored, and rots, which greatly reduces the commodity value of the fruit, increases the cost of disease and insect pest control for the fruit grower, and damages the economic benefits. [0003] At the present stage, it is usually controlled by spraying pesticides on citrus leaves, but on the one hand, pesticides are likely to make pathogenic bacteri...

Claims

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Application Information

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IPC IPC(8): C05G3/30C05G3/50C05G3/60
CPCC05C3/00C05G3/00
Inventor 吕昆明廖华廖博杨瑞青陆明光陆可敬吕贵升
Owner 广西乐土生物科技有限公司
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