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Toxic amanita species identification method based on DNA mini-barcode technology and application

A species identification and micro-barcoding technology, applied in the field of food quality and safety testing, can solve problems such as damage and inability to extract genes

Active Publication Date: 2020-08-25
CHINESE ACAD OF INSPECTION & QUARANTINE
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Problems solved by technology

But in some processed foods, the intact DNA cannot be extracted due to the damaged DNA ITS Gene, at this time extracted to part ITS Genes (100 bp-400 bp) can also accurately identify species

Method used

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  • Toxic amanita species identification method based on DNA mini-barcode technology and application
  • Toxic amanita species identification method based on DNA mini-barcode technology and application
  • Toxic amanita species identification method based on DNA mini-barcode technology and application

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Embodiment Construction

[0022] The present invention is further described by means of examples, but the present invention is not limited only to the following examples.

[0023] This example is to evaluate the accuracy of the amplification efficiency and identification of the DNA micro-barcode of the genus Amanita through the following tests.

[0024] 1. Experimental materials

[0025] The names of 43 mushroom samples of 33 species of Amanita used in this study are shown in Table 1. Among them, Pseudomonas amanita was provided by the Chinese Center for Disease Control and Prevention, and the rest of the samples of Amanita were provided by Professor Chen Zuohong of Hunan Normal University for morphological identification. ITS , Ribosomal large subunit (Large SubunitRibosomal, LSU ), the second largest subunit of RNA polymerase (the Second Largest Subunit of RNAPolymerase II, RPB2 ), β-tubulin ( β-tubulin ) 4 DNA barcodes to identify its species authenticity.

[0026] Table 1 Information of co...

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Abstract

The invention relates to the technical field of food quality and safety detection, and particularly discloses an amanita species identification method based on DNA mini-barcode and application. The method comprises the following steps: extracting toxic amanita genome DNA, then carrying out PCR amplification by utilizing self-designed DNA mini-barcode primers, sequencing a purified PCR product, comparing a DNA mini-barcode fragment sequence obtained by sequencing with a reference sequence in a public database, and carrying out species identification according to the matching similarity of the sequences. The method is simple and convenient to operate and high in accuracy, can identify an artificially simulated digested amanita sample, and has great application potential in analysis of amanita or vomit after mistaken eating and other scenes.

Description

technical field [0001] The invention belongs to the technical field of food quality and safety detection, and in particular relates to a method and application of a poisonous Amanita species identification method based on DNA micro-barcode technology. Background technique [0002] Amanita belongs to the kingdom Fungi ( Fungi ), Basidiomycotina ( Basidiomycotina ), Laminaria ( Hymenomycetes ), Agaricales ( Agaricales ), Amanitaceae ( Amanitace ), which is a large genus with a wide distribution among macrofungi. There are nearly 500 species of Amanita reported and verified in the world, and more than 130 species (subspecies, variants and variants) have been reported in my country. Among these amanitas, there are edible ones such as Amanita caesarea, Amanita caesarea ( Amanita ovoidea ), Red and Yellow Amanita ( Amanita hemibapha ), etc., there are also poisonous Amanita, such as Amanita cone scale ( Amanita virginoides ), Amanita spp. ( Amanita melleiceps ), A...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6895C12Q1/686C12Q2600/156C12Q2563/185
Inventor 陈颖邢冉冉白文明姬庆龙张九凯
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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