Mesenchymal stem cell drug for immune function regulation and preparation method thereof

Abstract

The invention discloses a mesenchymal stem cell drug for immune function regulation. The mesenchymal stem cell drug contains umbilical cord loose connective tissue mesenchymal stem cells (LCMSCs), wherein the LCMSCs are undifferentiated original mesenchymal stem cells extracted from loose connective tissues of a fetal and maternal connection interface in the umbilical cord of a newborn, and the LCMSCs are original undifferentiated mesenchymal stem cells derived from an extraembryonic mesoderm. The LCMSCs preserve the biological characteristics of the original MSCs to the maximum extent, and are far better in homing migration and differentiation and proliferation potentials than those of adult-derived MSCs, the colony forming unit of the LCMSCs is 300 times of that of bone-marrow-derived MSCs, secreted growth factors and nutritional factors are the most abundant, the secreted hepatocyte growth factor is 31.3 times of that of the BMSCs, various cancer suppressor genes can be highlightedand expressed, no tumor cell differentiation is caused by co-culture with various tumor cells in vitro, in-vivo fibroblast-like differentiation does not exist, immunomodulatory factors HGF, TSG-6, PGE2, MCP1, VEGFA, IL-6, IL-10, IDO, TGF-beta, sVCAM1 and the like can be highly expressed, the purity is far superior to that of MSCs derived from adults, umbilical cords, placentas, amnions and the like, and safety is high.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a mesenchymal stem cell medicine for regulating immune function and a preparation method thereof. Background technique [0002] Currently, the morbidity, disability, and mortality of ischemic heart disease, stroke, diabetes, various immune diseases, and degenerative diseases are still high. Severe lung injury-induced respiratory failure (ARDS) caused by the novel coronavirus pneumonia (COVID-2019) has claimed tens of thousands of lives. This is because the current medical field still lacks specific and effective treatment means for the above-mentioned diseases with few side effects. The above-mentioned diseases pose great challenges to modern medicine. [0003] In recent years, a large amount of scientific evidence has shown that mesenchymal stem cells (MSCs) with multi-directional differentiation and self-renewal play the roles of immune regulation, anti-inflammation, r...

AI Technical Summary

Problems solved by technology

[0005] Recently, some researchers isolated allogeneic MSCs from neonatal umbilical cord, umbilical blood, placenta, amniotic membrane and other tissues, but found that the content of MSCs in umbilical cord blood was small, while other impurity cells mixed in MSCs isolated from umbilical cord, placenta, and amniotic membrane Up to about 50-70%, seriously affecting the homing, proliferation, para-autocrine function, therapeutic effect and safety of MSCs

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