Erythrocytic cells and method for preserving cells

a technology of erythrocytic which is applied in the field of preservation and survival of human cells, eukaryotic cells and erythrocytic cells, can solve the problems of rapid loss of platelet function, limited shelf life under these conditions, and considerable pressure on blood transfusion centers to produce platelets, etc., to avoid platelet activation, preserve and/or increase the survival of dehydrated eukaryotic cells, the effect of increasing th
US20060134069A1Inactive Publication Date: 2006-06-22RGT UNIV OF CALIFORNIA
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
RGT UNIV OF CALIFORNIA
Publication Date
2006-06-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

A dehydrated composition is provided that includes freeze-dried erythrocytic cells. Alcohol (e.g., sterol or cholesterol) is at least partially removed from erythrocytic cells including erythrocytic membranes. After removal of at least part of the alcohol, the erythrocytic cells have a low phase transition temperature range, an intermediate phase transition temperature range, and a high phase transition temperature range. The erythrocytic cells may be loaded with an oligosaccharide (e.g., trehalose) which preserves biological properties during freeze-drying and rehydration. A process for increasing cooperativity of a phase transition of an erythrocytic cell. A process for preserving and / or increasing the survival of dehydrated erythrocytic cells, including storing dehydrated erythrocytic cells having a residual water content equal to or less than about 0.30 gram of water per gram of dry weight erythrocytic cells.
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Description

RELATED PATENT APPLICATIONS

[0001] This is a continuation-in-part patent application of copending patent application Ser. No. 09 / 927,760, filed Aug. 9, 2001. Patent application Ser. No. 09 / 927,760 is a continuation-in-part patent application of copending patent application Ser. No. 09 / 828,627, filed Apr. 5, 2001. Patent application Ser. No. 09 / 828,627 is a continuation patent application of patent application Ser. No. 09 / 501,773, filed Feb. 10, 2000. Benefit of all earlier filing dates is claimed with respect to all common subject matter.STATEMENT REGARDING FEDERAL SPONSORED RESEARCH AND DEVELOPMENT

[0002] Embodiments of this invention were made with Government support under Grant No. N66001-00-C-8048, awarded by the Department of Defense Advanced Research Projects Agency (DARPA). Further embodiments of this invention were made with Government support under Grant Nos. HL57810 and HL61204, awarded by the National Institutes of Health. The Government has certain rights to embodiments ...

Claims

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