Application of tea gamma-amino butyric acid in tumor radiotherapy
An aminobutyric acid, radiotherapy technology, applied in antitumor drugs, medical preparations containing active ingredients, radiation sensitizing materials, etc. therapeutic effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] Embodiment 1 human lung cancer cell line NCI-H292 cell line cell culture
[0039] Human lung cancer cell line NCI-H292 was cultured in RMPI-1640 culture medium containing 10% fetal bovine serum in 5% CO2 , cultured in a 37°C incubator, and when the cells entered the logarithmic growth phase, they were washed twice with PBS solution, and then digested with 0.25% trypsin. Continue culturing in the plate or petri dish to prepare for subsequent experiments. During cell culture, place the adherent cells at 37°C, saturated humidity, 5.0% CO 2 Continue to grow in the incubator.
Embodiment 2
[0040] Example 2 Determination of Intracellular Lactic Acid Content
[0041] Add γ-aminobutyric acid solution (the final concentration of γ-aminobutyric acid is 20mmol / L) to the culture dish of adherent NCI-H292 human lung cancer cells cultured under normoxia, and add an equal volume of PBS to the control group. After culturing for 48 hours, the medium was sucked off, washed twice with PBS, and then digested with 0.25% trypsin. After the digestion was terminated, the cells were centrifuged at 1000 r / min for 5 minutes to obtain precipitated cells. Resuspend and wash the cells with PBS and centrifuge again, add pre-cooled saline to the EP tube containing the cells and place on ice for homogenization. The prepared homogenate was centrifuged at 3000 r / min at 4°C for 15 min, the supernatant was aspirated, and the lactic acid content was determined according to the instructions of the lactic acid assay kit (Nanjing Jiancheng Biotechnology Co., Ltd.).
[0042] The test results of in...
Embodiment 3
[0043] Determination of Angiotensin II Content in the Supernatant of Example 3
[0044] Inoculate 2 × 10 in a 24-well plate 5 Each well of NCI-H292 lung cancer tumor cells in good condition was added with 1 mL of culture medium. After the cells adhered to the wall, the experimental group and the control group were treated with 20 mmol / L γ-aminobutyric acid and an equal volume of PBS respectively, and cultured normally for 48 hours. Afterwards, the medium supernatant was sucked into a centrifuge tube, and the content of angiotensin Ⅱ in the supernatant was determined according to the instructions of the angiotensin Ⅱ detection kit (RayBiotech, USA).
[0045] Angiotensin Ⅱ kit detected angiotensin Ⅱ in cell culture results showed that under the above culture conditions, NCI-H292 lung cancer tumor cells produced angiotensin Ⅱ, and the content of angiotensin Ⅱ in the cell supernatant increased significantly with the extension of culture time . Adding 20mmol / L γ-aminobutyric acid...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More