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A chip for simulating pathological model of cerebral ischemia-reperfusion

A technology of cerebral ischemia-reperfusion and pathological models, applied in the field of microfluidics, can solve problems such as unethical and low success rate of drugs, and achieve convenient observation, cultivation and detection, real-time and accurate monitoring, verification of rationality and feasibility sexual effect

Active Publication Date: 2022-05-17
DALIAN UNIV OF TECH
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Problems solved by technology

The traditional drug screening for cerebral ischemia-reperfusion is to construct an ischemic model in rats using the suture method, which is unethical and the success rate of drug screening is low due to differences in species.

Method used

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  • A chip for simulating pathological model of cerebral ischemia-reperfusion
  • A chip for simulating pathological model of cerebral ischemia-reperfusion
  • A chip for simulating pathological model of cerebral ischemia-reperfusion

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Embodiment 1

[0029] A pathological model chip of cerebral ischemia-reperfusion based on microfluidic technology. The chip is mainly composed of four layers of chips. The overall effect is as follows figure 1 As shown, the specific bonding sequence is as attached to the instruction manual figure 2shown. The material of the first layer chip and the fourth layer chip is polymethyl methacrylate (PMMA), and the second layer chip and the third layer chip use polydimethylsiloxane (PDMS) as the processing material. The enlarged view of the connection between the channel of the culture solution in the microfluidic chip and the middle culture well is as follows: image 3 As shown, the size of each layer of chips is the same, the length and width are both 30mm, and the heights of the four layers of chips from top to bottom are 2mm, 4mm, 4mm, and 2mm. After the chip is packaged as a whole, a microfluidic chip with a length of 30 mm, a width of 30 mm and a height of 14 mm is finally formed. Between...

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Abstract

A chip for simulating a pathological model of cerebral ischemia-reperfusion belongs to the field of microfluidic technology. It mainly includes four layers. The first layer is the culture medium and the inoculated cell inlet. The porous membrane between the second layer and the second and third layers of chips jointly simulates the blood-brain barrier system. Each layer of the chip is equipped with liquid inflow or outflow. The channel, the porous membrane between the third-layer chip and the third- and fourth-layer chips jointly simulates the functional unit of brain tissue. There are fine holes between the three or four layers of chips. The invention has multi-cell co-cultivation ability, through the design of porous membrane and culture hole, it can realize the non-contact information exchange between multi-cells through the flow of culture fluid, and then simulate the process of human brain hypoxia and glucose deficiency, and then simulate the process of human brain ischemia , Reperfusion after cerebral ischemia.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, and relates to a microfluidic chip-based universal chip for cerebral ischemia-reperfusion pathological models. Background technique [0002] 1. Hazards of cerebral ischemia-reperfusion and overview of microfluidic chips [0003] The brain is the most sensitive organ of the human body to hypoxia. Brain tissue ischemia will cause damage to local brain tissue function. The degree of damage is related to the length of ischemia time and the amount of residual blood flow. Short-term incomplete ischemia will only cause reversible Prolonged total ischemia or severe ischemia can cause infarction. Cerebral ischemia-reperfusion can cause serious damage to the brain, mainly manifested in abnormal brain metabolism, brain dysfunction, and structural damage to the brain: ischemia-reperfusion can lead to abnormal brain metabolism, because cerebral ischemia can lead to intracellular ATP The lack of, Na +...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/34C12M1/04B01L3/00
CPCC12M23/16C12M23/20C12M29/10C12M29/00C12M41/46B01L3/5027
Inventor 关水骆航孙长凯许建强石文龙徐卫平
Owner DALIAN UNIV OF TECH
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