Chip for simulating cerebral ischemia re-filled pathologic model

Abstract

The invention relates to a chip for simulating a cerebral ischemia re-filled pathologic model and belongs to the technical field of microfluidics. The chip mainly comprises four layers, wherein a first layer serves as a culture solution and inoculated cell inlet, a second layer and a porous membrane between a second-layer chip and a third-layer chip jointly simulate a blood-brain barrier system, achip of each layer is provided with a liquid inflow or outflow channel, the third-layer chip and a porous membrane between the third-layer chip and a fourth-layer chip jointly simulate a brain tissuefunctional unit, the chips are similarly provided with liquid inflow or outflow channels, the fourth-layer chip serves as a culture solution outlet, and moreover, microchannels are arranged between the second-layer chip and the third-layer chip and between the third-layer chip and the fourth-layer chip. The chip has multicell co-culture capability; and through designing the porous membranes and culture holes, contactless information exchange among cells, which flow through a culture solution, among multiple cells can be achieved, then, an oxygen-glucose deprivation process of a human brain issimulated, and then, a cerebral ischemia process and cerebral ischemia re-filling of human are simulated.

Description

technical field [0001] The invention belongs to the technical field of microfluidics, and relates to a general chip for cerebral ischemia-reperfusion pathological models based on a microfluidic chip. Background technique [0002] 1. Cerebral ischemia-reperfusion hazards and overview of microfluidic chips [0003] The brain is the most sensitive organ in the human body to hypoxia. Brain tissue ischemia will lead to damage to local brain tissue function. The degree of damage is related to the length of ischemia time and the amount of residual blood flow. Short-term incomplete ischemia can only cause reversible damage. damage, and prolonged complete ischemia or severe ischemia can cause infarction. Cerebral ischemia-reperfusion will cause serious damage to the brain, mainly in the abnormal brain metabolism, brain dysfunction and structural damage of the brain: ischemia-reperfusion will lead to abnormal brain metabolism, because cerebral ischemia will lead to intracellular ATP ...

AI Technical Summary

Problems solved by technology

The traditional drug screening for cerebral ischemia-reperfusion is to construct an ischemic model in rats using the suture method, which is unethical and the success rate of drug screening is low due to differences in species.

Images