Low-temperature amylase strain and production method of low-temperature amylase

Abstract

The invention relates to the technical field of low-temperature amylase production methods, in particular to a low-temperature amylase strain and a production method of low-temperature amylase. The method comprise the following steps of S1, adding a mixture of corn flour, wheat flour, yeast extract, medium honey and peptone at a top inlet, then connecting a feeding pipe on an inert gas tank containing aeromonas media with a feeding port of a fermentation machine, enabling the aeromonas media to enter the fermentation machine, finally adding a proper amount of deionized water, and performing fermentation for 2-3 days at the temperature of 15-25 DEG C; and S2, when fermentation liquor is taken after fermentation, inserting an injection needle tube from the feeding port of the fermentation machine, at the moment, adjusting and driving a rubber circular ring to enable the bottom of a rubber cylinder to move upwards, and sucking out bacterial colonies by using the injection needle tube. According to the method, the contact opportunity of a solution in the rubber cylinder and air is greatly reduced, so that the fermentation liquor can be prevented from being polluted; and when the next batch of bacterial colonies are cultured, raw materials can be directly injected from the feeding port, so that continuous production can be realized.

Description

technical field [0001] The invention belongs to the technical field of low-temperature amylase production methods, in particular to a low-temperature amylase strain and a production method of low-temperature amylase. Background technique [0002] Amylase is a general term for a group of enzymes that can catalyze the hydrolysis of starch into glucose, maltose and other oligosaccharides. Low-temperature amylase is a relative concept. Compared with mesophilic amylase, its optimum reaction temperature is 20-30°C lower than that of mesophilic amylase, and it has certain enzyme activity at 0°C. Low-temperature amylase can effectively play a catalytic role at low temperature, so it has a wide application prospect in the field of bioengineering and solving the modern energy crisis. [0003] At present, there are too many studies on the influence of different production methods on enzyme activity. The patent No. CN1952115A proposes a low-temperature amylase strain, low-temperature a...

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Problems solved by technology

[0004] In order to make up for the deficiencies of the prior art and solve the problems of the large contact area between raw materials and air in the prior art, which makes the raw materials easily polluted and the fermentation box in the prior art cannot carry out continuous culture of colonies, a low-temperature amylase strain proposed by the present invention , the production method of low temperature amylase

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