Method for collecting microorganisms and genes, and method for extracting same
An extraction method and microorganism technology, which are applied to the capture method of microorganisms and genes and the field of extraction thereof, can solve the problems of complicated operation, recovery loss, operation error, etc.
Active Publication Date: 2020-09-11
ZENSHO
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no. 1 Embodiment approach
[0087] Hereinafter, the method of this embodiment is demonstrated concretely using drawing.
[0088] figure 2 It is a schematic diagram which shows the flow of the 1st process and the 2nd process of this embodiment. Such as figure 2 As shown, the microtube 10 (first container) is composed of a test tube body 2 and a lid 4 . An opening 8 is provided at one end of the test tube main body 2 (on the upper side of the drawing), and the microtube 10 can be sealed by attaching the lid 4 to the opening 8 . Such as figure 2 As shown, the test tube main body 2 has an opening 8 at one end, and the other end (top end: lower side of the drawing) is closed by a wall. In addition, the test tube main body 2 has a cylindrical portion with a constant length and the same diameter from the opening 8 side toward the distal end, and further has a tapered portion that decreases in diameter toward the distal end. The other end of the test tube main body 2 forms the tip of the microtube 10, an...
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Abstract
Provided are a method for collecting microorganisms and genes, and a method for extracting same. The present invention includes: a first step in which a collection instrument provided with an absorbent body and a rod-like member in which a tip part having the absorbent body on one end thereof and a carrying part can be separated is used to cause a liquid medium to be absorbed into the absorbent body of the collection instrument; a second step in which the absorbent body of the collection instrument is brought into contact with a surface to be inspected to collect microorganisms or genes on thesurface to be inspected; a third step in which a first container, which has an opening on one end thereof and is sealable by attaching a lid body to the opening, is used, the collection instrument isinserted from the absorbent body side into the opening of the first container, the carrying part is withdrawn from the first container by separating the tip part and the carrying part of the rod-likemember after the collection instrument has been inserted, and the first container, in which the tip part is stored, is sealed so as to maintain the insertion direction; and a fourth step in which a second container, which has an opening on one end thereof and is sealable by attaching a lid body to the opening, is used, the tip part of the collection instrument is inserted from the first containerinto the second container so that the tip part is inserted from a side opposite to the absorbent body into the opening of the second container, and the second container, in which the tip part is stored, is sealed so as to maintain the insertion direction.
Description
technical field [0001] The invention relates to a wipe-type detection implemented in the food field, medical field, etc., and relates to a method for capturing microorganisms and genes and an extraction method thereof. Background technique [0002] Conventionally, in the food field, from the standpoint of safety and sanitation, swabbing tests for bacteria and viruses (hereinafter sometimes referred to as "microorganisms") have been performed in work spaces. In this test, a wiping test method is implemented, which uses a cotton swab or the like soaked with a predetermined amount of diluent such as phosphate buffer solution or physiological saline, and wipes the cooking utensils (such as chopping boards, kitchen knives, etc.) in the working space. , Other refrigerators, sinks, shelves, door handles, handrails, floors and other equipment, including microorganisms that exist on the surface of the test object, etc., and use methods such as culture to detect the microorganisms and...
Claims
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IPC IPC(8): G01N1/04G01N1/10
CPCG01N1/10G01N1/04G01N1/12
Inventor 小板桥努
Owner ZENSHO