A lncRNA marker related to porcine muscle fiber type and its application

A muscle fiber type and marker technology, applied in the field of livestock molecular marker preparation, can solve the problem of pork quality reduction

Active Publication Date: 2022-07-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, my country's commercial pigs are mainly lean-meat pig breeds imported from abroad, with fast growth, high lean meat rate and high feed conversion rate, but the quality of pork is significantly reduced

Method used

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  • A lncRNA marker related to porcine muscle fiber type and its application
  • A lncRNA marker related to porcine muscle fiber type and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Types of porcine muscle fibers of different breeds and high-throughput lncRNA-seq sequencing analysis

[0039] 1) Landrace pigs and Guangdong characteristic breed Lantang pigs with large differences in carcass muscle fiber types were selected as the research objects; 3 one-day-old Landrace pigs and 3 one-day-old Lantang pigs were selected to obtain their back muscle tissue and tissue specimens respectively. After being taken out, it was snap-frozen in liquid nitrogen for 30 seconds, and then stored in a -80°C refrigerator. Then, a part was subjected to paraffin-embedded sections and muscle fiber hematoxylin-eosin staining analysis, and the other part was sent to a sequencing company with dry ice for lncRNA sequencing.

[0040] 2) The apparent differences of muscle fibers in the back muscle tissue of one-day-old lean landrace pigs and fat bluetang pigs were significantly different. The number of muscle fiber nuclei per unit area of ​​Lantang pigs was significant...

Embodiment 2

[0046] Example 2 Fluorescence quantitative PCR detection of LincRNA-ROMT expression in porcine muscle tissue

[0047] Reagents:

[0048] Reverse Transcription Kit: PrimeScript TM RT reagent Kit with gDNA Eraser (Perfect RealTime), #RR047Q, Takara;

[0049] Quantitative PCR reagents: Fast qPCR Mix, #RR430A, Takara.

[0050] Relevant experimental items to RNase treatment:

[0051] 1) Rinse and soak all glassware with DEPC before application, 120 ℃ high pressure for 20 minutes, and 180 ℃ high temperature drying for more than 2 hours.

[0052] 2) Soak the plastic utensils (eg: EP tube / pipe tip) with 0.1% DEPC water overnight before use, then control the liquid to dry, press 120°C for 20 minutes, and bake it in an oven for use.

[0053] Total RNA extraction:

[0054] 50 cases of Landrace pig muscle tissue and 50 cases of Lantang pig muscle tissue were selected, and the TRIzol method was used according to the instructions of the Invitrogen kit. The specific steps are as follo...

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Abstract

The invention discloses a lncRNA marker related to porcine muscle fiber types and an application thereof, and belongs to the technical field of livestock molecular marker preparation. The lncRNA marker is LincRNA-ROMT, and its sequence is shown in SEQ ID NO: 1. The LincRNA‑ROMT is closely related to the type of porcine muscle fibers, and its expression is extremely low in oxidized porcine muscle fibers (fatty Lantang pig muscle tissue); it can be applied to the process of assisted genetic selection in pig breed improvement. The LincRNA-ROMT disclosed in the invention can be used as a molecular marker related to the type of porcine muscle fibers, and has important practical application value in genetically assisted breeding.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker preparation, and in particular relates to a lncRNA marker related to the type of porcine muscle fibers and its application. Background technique [0002] The improvement of muscle formation and quality traits of livestock and poultry is an important research direction of animal genetics and breeding. At present, my country's commercial pigs are mainly lean-meat pig breeds imported from abroad, with fast growth rate, high lean meat rate and feed conversion rate, but the pork quality is significantly reduced. In recent years, with the increasing demand of consumers for high-quality meat, there is an urgent need to cultivate high-quality pigs and improve meat quality. Muscle fiber is the basic unit of muscle tissue, and the type of muscle fiber is closely related to muscle quality, especially the tenderness of edible quality traits. Therefore, at the level of pig ketone body musc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/158C12Q2600/124C12Q2600/178C12Q2563/107C12Q2545/101C12Q2531/113
Inventor 孙加节张永亮江青艳习欠云陈婷罗君谊
Owner SOUTH CHINA AGRI UNIV
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