Tumor cell culture information analysis method
A technology for tumor cell and information analysis, which is applied in the fields of analysis materials, biochemical equipment and methods, and material separation, and can solve problems such as the difficulty in studying differences in phospholipid composition
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0025] S1. Tumor cell culture, provide tumor cells as starting cells, the tumor cells come from the same tumor tissue of the same subject, put the tumor cells into the culture medium for culture, and divide the culture samples into Class I, Class II and Class III, As a follow-up information comparison;
[0026] S2. Pharmacological culture. First, vascular endothelial cells are seeded on the osmotic support membrane of the culture container to form a vascular endothelial cell layer; the initial cells are placed on the osmotic support membrane vascular endothelial cell layer and form a semi-solid gel-like mixture. For the first, second and third types of culture, add anti-tumor drugs to the samples, collect the cell fluid in the culture container after culture, and count the cells. According to the number of cells in the culture container, the strength of the cell migration ability can be detected. weak;
[0027] S3. Cell membrane phospholipid extraction. After the tumor cell c...
Embodiment 2
[0032] A method for analyzing tumor cell culture information, comprising the steps of:
[0033] Step 1. Tumor cell culture, provide tumor cells as starting cells, the tumor cells come from the same tumor tissue of the same object, put the tumor cells into the medium for culture, and divide the culture samples into class I, class II and class III , as a follow-up information comparison, Class I, Class II and Class III correspond to early cells, mid-stage cells and late cells of tumor cells respectively, and the medium consists of 10% acellular ascites, 3% fetal bovine serum, 1% double Antibody, the rest is composed of DMEM medium;
[0034] Step 2, pharmacological culture, first inoculate vascular endothelial cells on the osmotic support membrane of the culture container to form a vascular endothelial cell layer; the osmotic support membrane is made of organic materials, and the initial cells are placed above the vascular endothelial cell layer of the osmotic support membrane an...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More