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Detection kit for detecting antibodies of cyclin D1 and BCL-2

A detection kit, BCL-2 technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of non-specific effects on tumor prevention and diagnosis

Pending Publication Date: 2020-09-18
JINLIN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it can be used as a reference indicator for the prognosis of various tumors, but because of its non-specificity, it affects its preventive and diagnostic role in tumors

Method used

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  • Detection kit for detecting antibodies of cyclin D1 and BCL-2
  • Detection kit for detecting antibodies of cyclin D1 and BCL-2
  • Detection kit for detecting antibodies of cyclin D1 and BCL-2

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Experimental program
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Embodiment

[0034] Cyclin D1 antibody (purchased from Abcam), BCL-2 antibody (purchased from Santa Cruz Biotechnology), coating solution (pH9.6, 0.05M carbonate buffer), washing buffer (pH7.4, 0.15M PBS), diluent (1%BSA), stop solution (2M, H 2 SO4), substrate buffer (pH5.0, phosphate-citrate buffer), tetramethylbenzidine working solution; 96-well microplate plate; PLUS 384 automatic microplate reader (MDC, USA).

[0035] The experimental process of the preparation method of the high-throughput ELISA kit:

[0036] Step 1: Dilute the cyclinD1 and BCL-2 antibodies with carbonate buffer solution to a concentration of 5 μg / ml, add 100 μl per plate, and coat overnight at 4°C. After sealing, wash the plate three times, dry it in vacuum for 1 hour, pack it and store it at 4°C for later use;

[0037] Step 2. Dilute the cyclinD1 and BCL-2 samples to be tested with PBST+1%BSA at 1:50, add 100 μl of the diluted sample, cyclinD1 standard and BCL-2 standard to the above-mentioned microtiter plate, and...

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Abstract

The invention discloses a detection kit for detecting antibodies of cyclin D1 and BCL-2 (Bcl-2). The kit comprises a solid-phase carrier, and a cyclinD1 antibody, a BCL-2 antibody, a sample diluent, an enzyme-labeled antibody, an enzyme-labeled antibody diluent, a developing solution, a stop solution, a washing solution and a standard curve comparison table of a strong positive reference substancewhich coat the solid-phase carrier.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection kit for detecting cyclinD1 and BCL-2 antibodies. Background technique [0002] There are many diagnostic methods for cancer, but imaging detection methods are the most widely used clinically, including X-ray, ultrasound, CT and nuclear magnetic resonance, etc., which are widely used clinically. However, on the one hand, the above-mentioned detection methods all require the cancer to grow and proliferate to a certain volume, and can be diagnosed with the naked eye in imaging. On the other hand, imaging screening involves the accumulation of radiation doses, if too much, it is suspected of causing cancer. In addition, the high cost of CT and MRI will increase the financial burden of patients, and is not suitable for the general screening of cancer in various parts of the human body. Therefore, under the current technical conditions, imaging methods are not convenien...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/577G01N33/574G01N33/543
CPCG01N33/581G01N33/577G01N33/57488G01N33/54393G01N2333/82
Inventor 钟越齐玲王晓军沈楠陈飞儿王玮瑶陈雪
Owner JINLIN MEDICAL COLLEGE