In vitro culture method of Begonia cavaleriei Levl

A technology of in vitro culture and begonia, applied in the direction of culture medium, horticultural methods, botanical equipment and methods, etc., can solve the problems of unknown environmental adaptability of offspring, many restrictive conditions, slow reproduction, etc.

Active Publication Date: 2020-09-22
GUIZHOU BOTANICAL GARDEN (GUIZHOU INST OF HORTICULTURAL SCI GUIZHOU INST OF BOTANY)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) Reproduction is slow and there are many restrictions
[0007] (2) Cannot effectively maintain the genetic stability of Changgan Begonia
[0008] (3) The environmental adaptability of the obtained offspring is unknown
[0009] The difficulty in solving the above problems and defects is: the growth habit of Changgan Begonia has not been reported, and little is known about it. It is necessary to carefully explore its breeding conditions and exercise its adaptability to the environment

Method used

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  • In vitro culture method of Begonia cavaleriei Levl
  • In vitro culture method of Begonia cavaleriei Levl
  • In vitro culture method of Begonia cavaleriei Levl

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Embodiment Construction

[0024] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0025] Aiming at the problems existing in the prior art, the present invention provides a method for in vitro cultivation of Changgan Begonia, which will be described in detail below in conjunction with the accompanying drawings.

[0026] Such as figure 1 As shown, the in vitro culture method of Changgan Begonia provided by the invention comprises the following steps:

[0027] S101: Inoculate with leaves, use MS+2mg / L 6-BA+0.3mg / L NAA for primary culture;

[0028] S102: The callus is transferred to the subculture medium MS+2mg / L 6-BA+0.5mg / L NAA+0.4mg / L GA;

[0029] S103: Root culture in medium 1 / 2MS+0.2mg / L NAA;

[003...

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Abstract

The invention belongs to the technical field of in vitro culture, and discloses an in vitro culture method of Begonia cavaleriei Levl. Inoculation is performed with mature leaves or leaf stalks of begonia, and culture media are adopted to perform primary culture so as to obtain calluses; the calluses are transferred to subculture media to perform differentiation to obtain adventitious buds; the adventitious buds are transferred to rooting media to culture so as to obtain sterile tissue culture seedlings; and the tissue culture seedlings are transferred to cultivation media to perform seedlinghardening. The utilization of leaves for the inoculation of primary culture is better, and the proportioning effects can be more ideal by selecting MS+2 mg/L6-BA+0.3 mg/L NAA; when the subculture media are MS+2 mg 6-BA+0.5 mg/L NAA+0.4 mg/L GA, the differentiation rate is the highest; the optimal choice of the rooting media is 1/2 MS+0.2 mg/L NAA, more roots can be achieved, the roots can be strong and developed, and the leaves of plants can be large and dense; and as the cultivation media are prepared by matching perlite, vermiculite and sphagnum moss, ideal hydrophobicity and water retentioncapacity can be simultaneously achieved, and the growth of the Begonia cavaleriei Levl can be promoted.

Description

technical field [0001] The invention belongs to the field of in vitro culture technology, and in particular relates to an in vitro culture method of Changgan begonia. Background technique [0002] Changgan Begonia (scientific name: Begonia cavaleriei Levl) is a perennial herbaceous plant belonging to the family Begoniaceae. Napo), Yunnan (Funing, Xichou, Malipo) and other places. Wild Changgan begonias grow in rocks and stone walls or crevices in the shade of ravines, under dense forests at the foot of mountains, and under dense forests in humid valleys. The altitude is 300-1000 meters, and the habitat is relatively fragile. [0003] The leaves of Changgan begonia are emerald green, peltate, basal, thick paper, with very short hairs on the top and nearly glabrous on the bottom. The petiole is 7-25 cm long and has ribs and glabrous; the flowering period is from April to July, and the flowers are white, Pale pink, pink, 1-3 flowers, cymose, petite and cute. It can be seen t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/15A01G24/28A01G22/60
CPCA01H4/001A01H4/005A01H4/008A01G24/15A01G24/28A01G22/60Y02P60/21
Inventor 任启飞马菁华欧明烛杨朔袁茂琴刘芳胡毅
Owner GUIZHOU BOTANICAL GARDEN (GUIZHOU INST OF HORTICULTURAL SCI GUIZHOU INST OF BOTANY)
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