In vitro culture method of Begonia cavaleriei Levl

Abstract

The invention belongs to the technical field of in vitro culture, and discloses an in vitro culture method of Begonia cavaleriei Levl. Inoculation is performed with mature leaves or leaf stalks of begonia, and culture media are adopted to perform primary culture so as to obtain calluses; the calluses are transferred to subculture media to perform differentiation to obtain adventitious buds; the adventitious buds are transferred to rooting media to culture so as to obtain sterile tissue culture seedlings; and the tissue culture seedlings are transferred to cultivation media to perform seedlinghardening. The utilization of leaves for the inoculation of primary culture is better, and the proportioning effects can be more ideal by selecting MS+2 mg / L6-BA+0.3 mg / L NAA; when the subculture media are MS+2 mg 6-BA+0.5 mg / L NAA+0.4 mg / L GA, the differentiation rate is the highest; the optimal choice of the rooting media is 1 / 2 MS+0.2 mg / L NAA, more roots can be achieved, the roots can be strong and developed, and the leaves of plants can be large and dense; and as the cultivation media are prepared by matching perlite, vermiculite and sphagnum moss, ideal hydrophobicity and water retentioncapacity can be simultaneously achieved, and the growth of the Begonia cavaleriei Levl can be promoted.

Description

technical field [0001] The invention belongs to the field of in vitro culture technology, and in particular relates to an in vitro culture method of Changgan begonia. Background technique [0002] Changgan Begonia (scientific name: Begonia cavaleriei Levl) is a perennial herbaceous plant belonging to the family Begoniaceae. Napo), Yunnan (Funing, Xichou, Malipo) and other places. Wild Changgan begonias grow in rocks and stone walls or crevices in the shade of ravines, under dense forests at the foot of mountains, and under dense forests in humid valleys. The altitude is 300-1000 meters, and the habitat is relatively fragile. [0003] The leaves of Changgan begonia are emerald green, peltate, basal, thick paper, with very short hairs on the top and nearly glabrous on the bottom. The petiole is 7-25 cm long and has ribs and glabrous; the flowering period is from April to July, and the flowers are white, Pale pink, pink, 1-3 flowers, cymose, petite and cute. It can be seen t...

AI Technical Summary

Problems solved by technology

[0006] (1) Reproduction is slow and there are many restrictions

[0007] (2) Cannot effectively maintain the genetic stability of Changgan Begonia

[0008] (3) The environmental adaptability of the obtained offspring is unknown

[0009] The difficulty in solving the above problems and defects is: the growth habit of Changgan Begonia has not been reported, and little is known about it. It is necessary to carefully explore its breeding conditions and exercise its adaptability to the environment

Images