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Application of sulforaphane in improving reproductive performance of mammals

A technology of sulforaphane and mammals, applied in the field of reproductive biology, can solve the problems of promoting the reproductive performance of mammals, achieve broad application prospects and commercial value, promote proliferation, and increase the weight of ovaries

Pending Publication Date: 2020-09-22
南京致润生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of the application of sulforaphane, many patents focus on its protective effect on adult health, and there is no patent on promoting the reproductive performance of mammals, such as the use of sulforaphane in inhibiting telomerase activity and preparing anticancer drugs (CN109771409A ), the application of sulforaphane in the preparation of food, health products or medicines for preventing diabetic lesions (CN108743576A), the application of sulforaphane in the preparation of drugs for the protection of fetal blood vessel development of women who drink alcohol during pregnancy (CN108771669B), etc.

Method used

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  • Application of sulforaphane in improving reproductive performance of mammals
  • Application of sulforaphane in improving reproductive performance of mammals
  • Application of sulforaphane in improving reproductive performance of mammals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Sulforaphane promotes the proliferation and vitality of granulosa cells in vitro, and inhibits the apoptosis of granulosa cells

[0029] The growth and development of follicles in the ovary is the physiological basis for estrus and ovulation in female mammals, and granulosa cells play a key role in the growth and development of follicles. Pig and mouse granulosa cells were cultured in vitro, and treated with different concentrations of sulforaphane (0 μM, 1 μM, 5 μM, 10 μM and 0 μM, 1 μM, 5 μM, 10 μM, 50 μM) for 24 hours to detect the changes in the viability of granulosa cells. In mice, the results of CCK-8 detection showed that low concentration of sulforaphane (1 μM) can significantly increase the viability of granulosa cells, while high concentrations of sulforaphane (5 μM, 10 μM, 50 μM) can actually inhibit the viability of granulosa cells ; In pigs, low concentrations of sulforaphane (1μM, 5μM) can also significantly increase the viability of granulosa c...

Embodiment 2

[0030] Example 2 Sulforaphane promotes the production of estradiol in vitro and the expression of steroid synthase genes such as StAR and CYP19A1 in granulosa cells

[0031] Steroid hormones play a crucial role in the proliferation and differentiation of granulosa cells, and it has been reported that estrogen can significantly promote the proliferation of granulosa cells (Saswati Das et al., 1996). Therefore, we detected the changes of estrogen levels and steroid hormone synthase in granulosa cells treated with low concentration of sulforaphane (1 μM). We collected the culture supernatant of granulosa cells after SFN treatment for 12h, and detected the estrogen level by ELISA, and found that the estrogen level in the SFN group was significantly increased ( figure 2 .A). The transcription and protein levels of steroid hormone synthase were detected by QRT-PCR and Western blot technology, and it was found that StAR was significantly up-regulated at the mRNA and protein levels,...

Embodiment 3

[0032] Example 3 Sulforaphane Promotes the Expression of FSH Receptor in Granulosa Cells in Vitro

[0033] The growth and development of follicles and the proliferation and differentiation of granulosa cells are controlled by the gonadotropins secreted by the pituitary and the sex steroid hormones secreted by the ovary itself. The follicle-stimulating hormone (FSH) secreted by the pituitary can significantly promote the proliferation of granulosa cells and can promote Granulosa cells produce FSHR and enhance the ability to respond to FSH, thus continuously promoting the development of follicles. In order to detect whether SFN can promote the expression of FSH receptors, improve the ability of granulosa cells to respond to FSH, and then promote the proliferation of granulosa cells, we also treated granulosa cells with low concentration of sulforaphane (1μM) for 12 hours, and then detected them by Western blot. The expression of FSH hormone receptor FSHR in granulosa cells was f...

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Abstract

The invention relates to application of sulforaphane in improving reproductive performance of mammals. The sulforaphane can promote proliferation and activity of in-vitro granulocytes, and inhibit apoptosis of the granulocytes. The sulforaphane promotes the generation of estradiol in vitro and the expression of steroid synthase genes such as granular cells StAR and CYP19A1. The sulforaphane promotes the expression of an in-vitro granulocyte FSH receptor. The sulforaphane promotes ovarian development of mice and increases ovarian weight. The sulforaphane promotes proliferation of granular cellsin vivo. The sulforaphane promotes development of ovarian follicles of mice, increases the number of follicles and increases the ovulation number. The sulforaphane is added into the daily ration forthe weaned sows, so that the litter size of the sows is remarkably increased. According to the technology for improving the reproductive performance of mammals by applying the sulforaphane, the new application of the sulforaphane is provided, the application value of the sulforaphane is greatly improved, the application range of the sulforaphane is expanded, the new field of applying the sulforaphane is expanded, and the sulforaphane has wide application prospect and commercial value.

Description

technical field [0001] The invention belongs to the technical field of reproductive biology, and more specifically relates to the application of sulforaphane in improving the reproductive performance of mammals. Background technique [0002] Reproduction is fundamental to the continuation of human generations. Unfortunately, human reproductive disorders frequently occur in today's society. According to statistics, about 10% of couples are troubled by reproductive disorders. On the other hand, the reproductive performance of livestock is an important economic trait. The double-lamb rate of sheep and other livestock and increasing the litter size of sows (shortening the gap with developed countries) are of great significance for improving the quality and efficiency of animal husbandry. Therefore, the research and development of new technologies to safely and efficiently improve the reproductive performance of mammals not only has great practical needs, but also has great appli...

Claims

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Application Information

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IPC IPC(8): A61K31/26A61P15/08
CPCA61K31/26A61P15/08
Inventor 刘红林陶景丽许波张轩周佳奇张良良李伟建李荣阳
Owner 南京致润生物科技集团有限公司