Research method for researching function of RBM8A gene for prompting glioblastoma proliferation

A technology for glioblastoma and cell proliferation, which is applied in the field of genetic engineering and can solve problems that have not yet been researched, analyzed and explored

Pending Publication Date: 2020-09-29
南宁市第一人民医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In summary, the existing problem is that we found that RBM8A can promote the proli

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  • Research method for researching function of RBM8A gene for prompting glioblastoma proliferation
  • Research method for researching function of RBM8A gene for prompting glioblastoma proliferation
  • Research method for researching function of RBM8A gene for prompting glioblastoma proliferation

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Embodiment Construction

[0028] In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with the examples. The specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0029] In the prior art, the specific mechanism by which RBM8A affects the proliferation of glioblastoma is still unclear. In order to solve the above technical problems, the present invention will be described in detail below in conjunction with specific analysis. The specific mechanism by which RBM8A promotes the proliferation of glioblastoma has not been clearly reported. Therefore, the present invention selects the RBM8A gene as the main analysis content. The present invention preliminarily determines that RBM8A is expressed in glioblastoma. On this basis, the present invention intends to further clarify the specific mechanism of RBM8A affecting t...

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Abstract

The invention relates to a research method for researching a function of an RBM8A gene for promoting glioblastoma proliferation. The method comprises the steps of 1) Western blot analysis of glioblastoma cell line RBM8A expression, 2) slow virus mediated knockout and overexpression of RBM8A, 3) effect of interfering RBM8A gene expression on glioblastoma cell proliferation, 4) observation of effectof knockout of RBMBA on glioblastoma proliferation at the animal level, 5) detection of the conditions of Notchl, phosphorylated STAT3 (p-STAT3), total STAT3 (STAT3), phosphorylated H3 (p-H3), totalH3 (H3) and actin in the glioblastoma cell line which interferes with RBM8A gene expression through Western blot analysis. The Notch/STAT3 pathway is a RBM8A-mediated cell proliferation mechanism of glioblastoma cells. The invention provides a new idea for treating the glioblastoma, and provides a new way for researching drug treatment targets of the glioblastoma.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a molecular mechanism and an early warning method for interfering with RBM8A gene expression to cause glioblastoma proliferation. Background technique [0002] Glioblastoma multiforme, also known as glioblastoma, is a highly malignant brain tumor with a very poor prognosis. The mortality rate of glioblastoma remains high, and the five-year survival rate of most patients is between 3% and 9% [Omuro A, Deangelis L M. Glioblastoma and Other Malignant Gliomas A Clinical Review [J]. JAMA The Journal of the American Medical Association, 2013, 310(17):1842-1850.]. Therefore, identifying new markers related to glioblastoma will help in the early diagnosis and treatment of glioblastoma. [0003] Aberrant expression of key factors in the nonsense-mediated mRNA degradation (NMD) pathway is associated with glioblastoma. Loss of NMD function can promote tumor growth and invasion[...

Claims

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Application Information

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IPC IPC(8): C12N15/867C12N5/10C12Q1/02
CPCC12N15/86C07K14/47C12N5/0693G01N33/5005C12N2740/15043
Inventor 邹东华林燕徐宁王浩李荣杰覃刚胡北泉
Owner 南宁市第一人民医院
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