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A kind of c-line antibody suitable for colloidal gold mixed labeling with specific pH and its application

A technology of colloidal gold and antibodies, applied in the direction of anti-animal/human immunoglobulin, instruments, measuring devices, etc., can solve the problems of loss of sensitivity, out-of-synchronization of immune gold activity status, inability to indicate the immune gold activity status, etc., to increase The effect of growth and decline transformation

Active Publication Date: 2022-04-26
北京纳百生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The color intensity of the false C-line is constant, and there is no ups and downs between the strength of the T-line and the C-line, which loses part of the sensitivity
In addition, the false C line has nothing to do with the activity of colloidal gold markers, and is not synchronized with the activity state of immunogold, so it cannot indicate the activity state of immunogold, and it is easy to cause false positives in quality control

Method used

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  • A kind of c-line antibody suitable for colloidal gold mixed labeling with specific pH and its application
  • A kind of c-line antibody suitable for colloidal gold mixed labeling with specific pH and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0022] This embodiment provides a C-line antibody suitable for colloidal gold mixed labeling at a specific pH, the C-line antibody is a casein antibody, and its preparation and screening method includes the following steps:

[0023] (1) Casein was selected as the immunogen; among them, casein and other blocking proteins are abundant in natural samples, easy to obtain, and have good specificity, basically no cross-reaction with other detection substances, and have the characteristics of easy acquisition, Low cost and other advantages.

[0024] (2) Inject casein into mice, and perform four immunizations: first immunization, second immunization, third immunization, and booster immunization; the specific immunization procedures are as follows:

[0025] First immunization: on D0 day, casein and Freund's complete adjuvant were mixed and injected into the abdominal cavity of mice, with a dose of 50 mg;

[0026] Second immunization: D21 days, casein mixed with Freund's incomplete adj...

Embodiment 2

[0037] This embodiment provides a sample mixture, including the sample to be labeled and the casein antibody provided in Example 1 above; the concentration of the casein antibody is 0.8 μg / mL. The concentration of the sample to be labeled is 4 μg / mL. The solvent of the sample mixture is water. Wherein, the sample to be labeled is the sample that actually needs to be tested for labeling; the preparation method of the casein antibody is the same as that in Example 1.

Embodiment 3

[0039]This embodiment provides a sample mixture, including the sample to be labeled and the casein antibody provided in Example 1 above; the concentration of the casein antibody is 1.5 μg / mL. The concentration of the sample to be labeled is 6 μg / mL. The solvent of the sample mixture is water. Wherein, the sample to be labeled is the sample that actually needs to be tested for labeling; the preparation method of the casein antibody is the same as that in Example 1.

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Abstract

The invention discloses a colloidal gold mixed labeling C-line antibody suitable for specific pH and application thereof, belonging to the technical field of immune colloidal gold. The C-line antibody is a casein antibody; the amino acid sequence of the heavy chain of the C-line antibody includes the sequence shown in SEQ ID NO: 1 in the sequence listing; the amino acid sequence of the light chain of the C-line antibody includes the sequence listing The sequence shown in SEQ ID NO:2. The C-line antibody can be prepared into a sample mixture with the sample to be labeled for immunocolloidal gold labeling, and the pH of the labeling is 8.3-9. In the present invention, by mixing the casein antibody with the sample to be labeled, the combination with the true C-line goat anti-mouse can be realized, thereby increasing the ups and downs of the T-line and C-line, so as to improve the sensitivity and sensitivity of lateral flow chromatography detection. Avoid false positives.

Description

technical field [0001] The invention relates to the technical field of immune colloidal gold, in particular to a colloidal gold mixed labeling C-line antibody suitable for a specific pH and application thereof. Background technique [0002] Immune colloidal gold technique (Immune colloidal gold technique) is an immune labeling technique that uses colloidal gold as a tracer marker to detect antigens and antibodies. The English abbreviation is: GICT. Colloidal gold is made from chloroauric acid (HAuCl 4 ) under the action of reducing agents such as ascorbic acid, sodium citrate, etc., reductively polymerize into gold nanoparticles of a specific size, and become a stable colloidal state due to electrostatic interaction, called colloidal gold. Colloidal gold particles are negatively charged and can form a strong bond with the positively charged groups of protein molecules. Since this bond is electrostatic, it does not affect the biological characteristics of the protein. In ad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/18G01N33/577G01N33/532G01N33/558
CPCC07K16/18G01N33/577G01N33/532G01N33/558
Inventor 杨春江袁志波马孝斌
Owner 北京纳百生物科技有限公司
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