A kind of c-line antibody for immunocolloidal gold mixed labeling and application thereof
A colloidal gold and antibody technology, applied in immunoglobulins, anti-animal/human immunoglobulins, instruments, etc., can solve the problems of loss of sensitivity, inability to indicate the active state of immunogold, false positives in quality control, etc. Transform effect
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Embodiment 1
[0022] This embodiment provides a C-line antibody for immunocolloidal gold mixed labeling, the C-line antibody is a bovine serum albumin antibody, and its preparation and screening method includes the following steps:
[0023] (1) Bovine serum albumin was selected as the immunogen; among them, bovine serum albumin and other blocking proteins are abundant in natural samples, easy to obtain, and have good specificity, basically no cross-reaction with other detection substances, and It has the advantages of easy acquisition and low cost.
[0024] (2) Inject bovine serum albumin into mice, and perform four immunizations: first immunization, second immunization, third immunization, and booster immunization; the specific immunization procedures are as follows:
[0025] First immunization: On D0 day, bovine serum albumin mixed with Freund's complete adjuvant was injected into the abdominal cavity of mice, with a dose of 50 mg;
[0026] Second immunization: On D21, bovine serum album...
Embodiment 2
[0037] This embodiment provides a sample mixture, including the sample to be labeled and the bovine serum albumin antibody provided in the above-mentioned embodiment 1; the concentration of the bovine serum albumin antibody is 0.8 μg / mL. The concentration of the sample to be labeled is 4 μg / mL. The solvent of the sample mixture is water. Wherein, the sample to be labeled is the sample that actually needs to be tested for labeling; the preparation method of the bovine serum albumin antibody is the same as that in Example 1.
Embodiment 3
[0039]This embodiment provides a sample mixture, including the sample to be labeled and the bovine serum albumin antibody provided in the above-mentioned embodiment 1; the concentration of the bovine serum albumin antibody is 1.5 μg / mL. The concentration of the sample to be labeled is 6 μg / mL. The solvent of the sample mixture is water. Wherein, the sample to be labeled is the sample that actually needs to be tested for labeling; the preparation method of the bovine serum albumin antibody is the same as that in Example 1.
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