A kind of c-line antibody for immunocolloidal gold mixed labeling and application thereof

Abstract

The invention discloses a C-line antibody for immunocolloidal gold mixed labeling and application thereof, belonging to the technical field of immunocolloidal gold. The C-line antibody is a bovine serum albumin antibody; the amino acid sequence of the heavy chain of the C-line antibody includes the sequence shown in SEQ ID NO: 1 in the sequence listing; the amino acid sequence of the light chain of the C-line antibody includes the following: The sequence shown in SEQ ID NO: 2 in the sequence listing. The C-line antibody can be prepared into a sample mixture with the sample to be labeled for immunocolloidal gold labeling, and the pH of the labeling is 7.8-8.4. In the present invention, by mixing the bovine serum albumin antibody with the sample to be labeled, the combination with the true C-line goat anti-mouse can be realized, thereby increasing the ups and downs of the T-line and the C-line, so as to improve the detection efficiency of lateral flow chromatography. Sensitivity and avoidance of false positives.

Description

technical field [0001] The invention relates to the technical field of immune colloidal gold, in particular to a C-line antibody for mixed labeling of immune colloidal gold and its application. Background technique [0002] Immune colloidal gold technique (Immune colloidal gold technique) is an immune labeling technique that uses colloidal gold as a tracer marker to detect antigens and antibodies. The English abbreviation is: GICT. Colloidal gold is made from chloroauric acid (HAuCl 4 ) under the action of reducing agents such as ascorbic acid, sodium citrate, etc., reductively polymerize into gold nanoparticles of a specific size, and become a stable colloidal state due to electrostatic interaction, called colloidal gold. Colloidal gold particles are negatively charged and can form a strong bond with the positively charged groups of protein molecules. Since this bond is electrostatic, it does not affect the biological characteristics of the protein. In addition to binding...

AI Technical Summary

Problems solved by technology

The color intensity of the false C-line is constant, and there is no ups and downs between the strength of the T-line and the C-line, which loses part of the sensitivity

In addition, the false C line has nothing to do with the activity of colloidal gold markers, and is not synchronized with the activity state of immunogold, so it cannot indicate the activity state of immunogold, and it is easy to cause false positives in quality control

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