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C line antibody for immune colloidal gold mixed labeling and application of C line antibody

A colloidal gold and antibody technology, applied in immunoglobulin, anti-animal/human immunoglobulin, biochemical equipment and methods, etc., can solve problems such as loss of sensitivity, inability to indicate the active state of immunogold, and false positives in quality control , to achieve the effect of increasing the ups and downs transformation

Active Publication Date: 2020-09-11
北京纳百生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The color intensity of the false C-line is constant, and there is no ups and downs between the strength of the T-line and the C-line, which loses part of the sensitivity
In addition, the false C line has nothing to do with the activity of colloidal gold markers, and is not synchronized with the activity state of immunogold, so it cannot indicate the activity state of immunogold, and it is easy to cause false positives in quality control

Method used

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  • C line antibody for immune colloidal gold mixed labeling and application of C line antibody
  • C line antibody for immune colloidal gold mixed labeling and application of C line antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This embodiment provides a C-line antibody for immunocolloidal gold mixed labeling, the C-line antibody is a bovine serum albumin antibody, and its preparation and screening method includes the following steps:

[0023] (1) Bovine serum albumin was selected as the immunogen; among them, bovine serum albumin and other blocking proteins are abundant in natural samples, easy to obtain, and have good specificity, basically no cross-reaction with other detection substances, and It has the advantages of easy acquisition and low cost.

[0024] (2) Inject bovine serum albumin into mice, and perform four immunizations: first immunization, second immunization, third immunization, and booster immunization; the specific immunization procedures are as follows:

[0025] First immunization: On D0 day, bovine serum albumin mixed with Freund's complete adjuvant was injected into the abdominal cavity of mice, with a dose of 50 mg;

[0026] Second immunization: On D21, bovine serum album...

Embodiment 2

[0037] This embodiment provides a sample mixture, including the sample to be labeled and the bovine serum albumin antibody provided in the above-mentioned embodiment 1; the concentration of the bovine serum albumin antibody is 0.8 μg / mL. The concentration of the sample to be labeled is 4 μg / mL. The solvent of the sample mixture is water. Wherein, the sample to be labeled is the sample that actually needs to be tested for labeling; the preparation method of the bovine serum albumin antibody is the same as that in Example 1.

Embodiment 3

[0039]This embodiment provides a sample mixture, including the sample to be labeled and the bovine serum albumin antibody provided in the above-mentioned embodiment 1; the concentration of the bovine serum albumin antibody is 1.5 μg / mL. The concentration of the sample to be labeled is 6 μg / mL. The solvent of the sample mixture is water. Wherein, the sample to be labeled is the sample that actually needs to be tested for labeling; the preparation method of the bovine serum albumin antibody is the same as that in Example 1.

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Abstract

The invention discloses a C line antibody for immune colloidal gold mixed labeling and application of the C line antibody, and belongs to the technical field of immune colloidal gold. The C line antibody is a bovine serum albumin antibody; the amino acid sequence of a heavy chain of the C line antibody is a sequence shown by SEQ ID NO:1 in a sequence table; and the amino acid sequence of a light chain of the C line antibody comprises a sequence shown by SEQ ID NO:2 in the sequence table. The C line antibody and a sample to be labeled can be prepared into a sample mixed solution for immune colloidal gold mixed labeling; and the labeling pH is 7.8 to 8.4. By mixing the bovine serum albumin antibody and the sample to be labeled, the bonding to a real C line goat-anti-mouse antibody can be realized, so that the intensity growth and decline conversion of a T line and a C line can be enhanced so as to improve the sensitivity of lateral tomography detection and to avoid the occurrence of a false positive result.

Description

technical field [0001] The invention relates to the technical field of immune colloidal gold, in particular to a C-line antibody for mixed labeling of immune colloidal gold and its application. Background technique [0002] Immune colloidal gold technique (Immune colloidal gold technique) is an immune labeling technique that uses colloidal gold as a tracer marker to detect antigens and antibodies. The English abbreviation is: GICT. Colloidal gold is made from chloroauric acid (HAuCl 4 ) under the action of reducing agents such as ascorbic acid, sodium citrate, etc., reductively polymerize into gold nanoparticles of a specific size, and become a stable colloidal state due to electrostatic interaction, called colloidal gold. Colloidal gold particles are negatively charged and can form a strong bond with the positively charged groups of protein molecules. Since this bond is electrostatic, it does not affect the biological characteristics of the protein. In addition to binding...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C12N5/20G01N33/558C12R1/91
CPCC07K16/18G01N33/558C07K2317/35C07K2317/33
Inventor 杨春江袁志波马孝斌
Owner 北京纳百生物科技有限公司
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