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Method for detecting effective action time and bactericidal efficacy of sterilization product

A technology of effective effect and detection method, which is applied in the field of microbial detection, can solve the problems of large differences in actual use results, uncontrollable environmental variables of bactericidal efficacy testing, and inconsistent carriers.

Pending Publication Date: 2020-10-09
林 弘毅
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the difficulty in accurately and quickly determining the effective time of action during the research and development of new bactericidal products, the uncontrollable environmental variables in the bactericidal efficacy test of simulated usage, the carrier is not uniform and difficult to operate, and the result error between repeated tests Large, uneven distribution of microorganisms, large differences between simulated test results and actual use results, and many other deficiencies

Method used

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  • Method for detecting effective action time and bactericidal efficacy of sterilization product
  • Method for detecting effective action time and bactericidal efficacy of sterilization product
  • Method for detecting effective action time and bactericidal efficacy of sterilization product

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specific Embodiment

[0163] Its specific embodiment: new household sterilization solution 091219, main components: alcohol (75%) sterile water, tea tree essential oil (0.05%). This product is positioned to disinfect the surface of household objects. According to the actual use environment of the product, the degree of organic interference in the use environment of this product is low, and the surface area of ​​the object is greater than 25 cm 2 , therefore, using 25 cm 2 The glass carrier kit is used for the spray action test, and the organic interfering agent is 0.3% bovine albumen serum. According to previous test results, TSB containing 0.6% Tween was used as neutralization solution and recovery medium, tryptic soy agar was used as medium, and Escherichia coli ( E. coli ATCC 10536) and Staphylococcus aureus ( S. aureus ATCC 6538) as the test strain.

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Abstract

The invention discloses a method for detecting effective action time and bactericidal efficacy of a sterilization product. The method comprises the following steps of: (1) selecting a required microbial carrier kit according to an actual action area before testing; (2) screening effective action time by using the carrier kit; (3) carrying out a unit area sterilization efficacy test by utilizing the carrier kit; and (4) calculating the sterilization efficacy per unit area by utilizing a formula and analyzing a test result. The method is suitable for research and development of sterilization products, the effective action time of the products can be rapidly determined, the sterilization efficacy per unit area in simulated use can be accurately detected, meanwhile, the matched carrier for thetest enables microorganism distribution to be more average, test variables to be closer to reality and environmental variables to be controllable, and therefore uncertainty of test results is reduced, and the reliability is improved. In addition, the scheme has the characteristics that the kit can be repeatedly used, the test operation is simple, the cost is low, the repetition is easy and the like, and is very beneficial to debugging and evaluating the performance of a new product in the research and development process of a novel sterilization product.

Description

technical field [0001] The invention relates to the technical field of microbial detection, in particular to a detection method applicable to the efficacy detection of new bactericidal products, which can quickly determine the effective action time of new bactericidal products and detect the bactericidal efficacy per unit area in simulated use. Background technique [0002] Pathogens are microorganisms that can cause infections in humans, including bacteria, fungi, and molds. Among the currently known 30,000 species of bacteria and 120,000 species of fungi, 5% of the species can be harmful to humans or even cause death. For example: There was a large-scale plague outbreak in Europe in the Middle Ages. At that time, because people had limited awareness of health, Yersinia pestis (Yersinia pestis) It spread to human settlements through fleas that lived on rodents, killing 25 million people and making it one of the deadliest plague events in human history. Until the 21st cent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G06F17/10
CPCC12Q1/04G06F17/10Y02A50/30
Inventor 林弘毅
Owner 林 弘毅
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