Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Vitis vinifera anther inducing culture medium capable of increasing inductivity of embryonic calluses

A technology of embryogenic callus and induction medium, which is applied in the field of plant tissue culture to achieve the effects of optimizing the culture method, improving the culture efficiency, and increasing the induction rate

Inactive Publication Date: 2020-10-13
江苏高航农业科技有限公司
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And lack a kind of special culture medium that can improve grape anther induction efficiency at present

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vitis vinifera anther inducing culture medium capable of increasing inductivity of embryonic calluses
  • Vitis vinifera anther inducing culture medium capable of increasing inductivity of embryonic calluses
  • Vitis vinifera anther inducing culture medium capable of increasing inductivity of embryonic calluses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1 test material

[0029] 1.1 Test materials

[0030] Two table grape varieties "Victoria" and "Gui Fei Rose" were used as anther culture materials.

[0031] 1.2 Medium

[0032] The formulation of the induction medium is as follows:

[0033] Macroelements: KNO 3 2650mg / L, (NH 4 ) 2 SO 4 120mg / L, NaH 2 PO 4 2H 2 O 215mg / L, CaCl 2 2H 2 O191mg / L, MgSO 4 160mg / L, KCl 97mg / L;

[0034] Trace elements: MnSO 4 4H 2 O 10.2mg / L, ZnSO 4 ·7H 2 O 2.0mg / L, H 3 BO 3 6.0mg / L, KI 0.75mg / L, Na 2 MoO 4 2H 2 O 0.5mg / L, CuSO 4 ·5H 2 O 0.025mg / L, CoCl 2 ·6H 2 O 0.025mg / L;

[0035] Iron salts and complexes: FeSO 4 ·7H 2 O 28.3mg / L, Na 2 -EDTA 37.4mg / L;

[0036] Organic ingredients: inositol 90mg / L, vitamin B1 8.5mg / L, vitamin B6 2.0mg / L, niacin 1.0mg / L, biotin 0.8mg / L, vitamin C 4.0mg / L, taurine 1.0mg / L L, Serine 2.0mg / L, Glycine 2.0mg / L, Phenylalanine 23mg / L, Fumaric Acid 36mg / L;

[0037] Other additional ingredients: casein hydrolyzate 105mg / L, thiourea 12.5m...

Embodiment 2

[0061] Effects of Different Induction Mediums on the Induction of Embryogenic Callus from Grape Anthers

[0062] Culture medium of the present invention: KNO 3 2650mg / L, (NH 4 ) 2 SO 4 120mg / L, NaH 2 PO 4 2H 2 O 215mg / L, CaCl 2 2H 2 O 191mg / L, MgSO 4 160mg / L, KCl 97mg / L, MnSO 4 4H 2 O 10.2mg / L, ZnSO 4 ·7H 2 O 2.0mg / L, H 3 BO 3 6.0mg / L, KI 0.75mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CuSO 4 ·5H 2 O 0.025mg / L, CoCl 2 ·6H 2 O 0.025mg / L, FeSO 4 ·7H 2 O 28.3mg / L, Na 2 -EDTA 37.4mg / L, Inositol 90mg / L, Vitamin B18.5mg / L, Vitamin B6 2.0mg / L, Niacin 1.0mg / L, Biotin 0.8mg / L, Vitamin C 4.0mg / L, Taurine Acid 1.0mg / L, Serine 2.0mg / L, Glycine 2.0mg / L, Phenylalanine 23mg / L, Fumaric Acid 36mg / L, Casein Hydrolyzate 105mg / L, Thiourea 12.5mg / L, Sodium Nitroprusside 50μmol / L, Sodium Naphthenate 1.0mg / L, Dicamba 1.0mg / L, 6-BA2.0mg / L, Grape Seed Powder 0.4g / L, Root Extract 60ml / L, Sucrose 30g / L, Plant Congeal Glue 6g / L.

[0063] Control Medium 1: B 5 +6-BA 4.0mg / L+2,4-D 0....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a vitis vinifera anther inducing culture medium capable of increasing inductivity of embryonic calluses. The formula comprises macroelements (KNO3, NH4NO3, KH2PO4, CaCl2.2H2O,Mg2SO4 and KCl), trace elements (KI, H3BO3, MnSO4.4H2O, ZnSO4.7H2O, Na2MoO4.2H2O, CuSO4.5H2O and CoCl2.6H2O), iron salt and complex compounds (FeSO4.7H2O and Na2-EDTA), organic components (inositol, vitamin B1, vitamin B6, niacin, biotin, vitamin C, taurine, serine, glycine, phenylalanine and fumaric acid), other additional components (casein hydrolysate, thiourea, sodium nitroprusside, sodium napthionate, Banex, 6-BA, vitis vinifera seed powder and a bryophyllum pinnatum extraction solution), a carbon source and a coagulator (cane sugar and plant gel). The vitis vinifera anther inducing culture medium disclosed by the invention can significantly increase the inductivity of embryonic calluses of vitis vinifera anthers, and has important significance in improving a vitis vinifera anther culture method and improving the vitis vinifera anther culture efficiency.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a grape anther induction medium capable of increasing the induction rate of embryogenic callus. Background technique [0002] Grapes (scientific name: Vitis vinifera L.) Also known as grass dragon ball, bodhi seed, mountain gourd, etc., it is a woody vine of the genus Vitis vinifera. In ancient my country, it was called "Pu Tao", "Pu grape", "Pu Yao" and "Pao Tao". One of the ancient fruits. Grapes are bright in color, sweet and sour, and have high nutritional value. They are loved by people and are called "crystal pearls". The sugar content of grapes is very high, mainly glucose, including fructose and sucrose. Grapes are rich in protein, multivitamins, carbohydrates, fat, dietary fiber, and various minerals such as calcium, magnesium, phosphorus, iron, manganese, etc., as well as a variety of amino acids and fruit acids needed by the human body. Grape is flat i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 邱振东梁璐
Owner 江苏高航农业科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com