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Efficient method for inducing somatic embryos of white-flower camellia oleifera abel Ganwu-2

A technology of camellia oleifera and white flower, which is applied in the field of high-efficiency somatic embryo induction of Camellia oleifera Ganwu-2, and can solve the problems of limited tissue culture characteristics, low somatic embryo induction rate, and low regeneration rate.

Active Publication Date: 2020-10-16
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Predecessors have accumulated some foundations in the establishment of somatic embryo induction and tissue culture systems of Camellia oleifera, but the somatic embryo induction rate is low, the regeneration rate is low, and it is severely limited by the tissue culture characteristics of different varieties.

Method used

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  • Efficient method for inducing somatic embryos of white-flower camellia oleifera abel Ganwu-2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This method comprises the following steps:

[0034] A. Explant Selection

[0035] Take fresh camellia oleifera fruit, remove the peel with a blade, cut the mature camellia oleifera embryo into 1cm long and 0.5cm wide pieces with a blade, wash with water for 3-5 times, and remove the oil stains secreted by the camellia oleifera embryo;

[0036] B. Disinfection of explants

[0037] Wash the small pieces of Camellia oleifera embryos twice with 75% alcohol, then sterilize them with 0.01% mercuric chloride for 20 minutes, and finally wash them with sterile water for 3-5 times, put all the camellia oleifera embryos on sterile filter paper for blowing. Dry;

[0038] C. Preparation of callus induction medium

[0039] Add 0.5mg / L 2,4-D, 0.1mg / L TDZ, 0.1mg / L KT, 250mg / L acid hydrolyzed casein, 30g / L sucrose, 6g / L agar, pH is 5.6;

[0040] D. Callus induction and subculture

[0041] Put the sterilized Camellia oleifera embryos on the callus induction medium for induction, pu...

Embodiment 2

[0045] This method comprises the following steps:

[0046] a. Explant selection

[0047] Take fresh camellia oleifera fruit, remove the peel with a blade, cut the mature camellia oleifera embryo into 1 cm long and 0.5 cm wide pieces with a blade, and wash with water for 3-5 times;

[0048] b. Disinfection of explants

[0049]Wash the small pieces of Camellia oleifera embryos twice with 75% alcohol, then sterilize them with 0.01% mercuric chloride for 30 min, and finally wash them with sterile water for 3-5 times, and put all the camellia oleifera embryos on sterile filter paper for sterilization. blow dry.

[0050] c. Preparation of callus induction medium

[0051] Add 2mg / L 2,4-D, 0.5mg / L TDZ, 0.5mg / L KT, 250mg / L acid hydrolyzed casein, 30g / L sucrose, 7g / L agar in sequence to the basic MS medium, the pH is 5.8;

[0052] d. Callus induction and subculture

[0053] Put the sterilized Camellia oleifera embryos on the callus induction medium for induction, put 10-15 explant...

Embodiment 3

[0058] This method comprises the following steps:

[0059] ⅰ. Explant Selection

[0060] Take fresh camellia oleifera fruit, remove the peel with a blade, cut the mature camellia oleifera embryo into 1 cm long and 0.5 cm wide pieces with a blade, and wash with water for 3-5 times;

[0061] ⅱ. Disinfection of explants

[0062] Wash the small pieces of Camellia oleifera embryos twice with 75% alcohol, then sterilize them with 0.01% mercuric chloride for 30 min, and finally wash them with sterile water for 3-5 times, and put all the camellia oleifera embryos on sterile filter paper for sterilization. blow dry;

[0063] ⅲ. Preparation of callus induction medium

[0064] Add 4mg / L 2,4-D, 6mg / L TDZ, 5mg / L KT, 750mg / L acid hydrolyzed casein, 30g / L sucrose, 9g / L agar in sequence to the basic MS medium, the pH is 6.0;

[0065] ⅳ. Callus induction and subculture

[0066] Put the sterilized Camellia oleifera embryos on the callus induction medium for induction, put 10-15 explants in...

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Abstract

The invention discloses an efficient method for inducing somatic embryos of white-flower camellia oleifera abel Ganwu-2, and relates to methods for inducing the somatic embryos of the white-flower camellia oleifera abel in the technical field of biology. The method comprises the following steps of (1), selecting explants; (2), disinfecting the explants; (3), preparing a callus induction culture medium; (4), performing callus induction and subculture; and (5), performing somatic embryo induction. According to a formula of the culture medium, the mature embryos of the camellia oleifera abel serve as the explants, and a system for inducing the somatic embryos of the white-flower camellia oleifera abel Ganwu-2 is established for the first time; the system for inducing the somatic embryos of the white-flower camellia oleifera abel Ganwu-2 is efficiently established, wherein multiple indexes are higher than those of the same kind of white-flower camellia oleifera abel; and on the basis, a certain foundation is laid for overcoming a genetic transformation system of the white-flower camellia oleifera abel, and the development of camellia oleifera abel breeding career is better promoted.

Description

technical field [0001] The invention relates to a method for inducing somatic embryos of Camellia oleifera in the field of biotechnology, in particular to an efficient method for inducing somatic embryos of Camellia oleifera Ganwu-2. Background technique [0002] Camellia ( Camellia oleifera Abel) belongs to the genus Camellia, is a kind of economic crop with high oil content, and is widely planted in southern my country. According to the classification of flowers and colors, camellia oleifera can be divided into red flower camellia, white flower camellia and yellow flower camellia. Camellia oleifera belongs to cross-pollination plants, and predecessors have bred more than 10 varieties that can be used for food through cross-breeding methods. White-flowered camellia oleifera is an important cultivar, which has the characteristics of high content and firmness, so it is deeply loved by the majority of camellia oleifera growers. The breeding method of camellia oleifera only...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/06
CPCA01H4/001A01H4/008A01H1/06
Inventor 姚瑞枫郭新红袁建波张蒙陈丽陈丰林
Owner HUNAN UNIV
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