Reinforcing agent for anti-tumor effect of polymer type anti-cancer drug
A polymer-based, anti-cancer drug technology, applied in anti-tumor drugs, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc. Insufficient effect, etc.
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[0082] The following examples illustrate the present invention. However, the present invention is not interpreted as being limited to the following Examples.
[0083] 1. Experimental materials and methods
[0084] 1-1. Cell culture
[0085] In the experiments shown below, two human colorectal cancer cell lines, HCT116 and HT29, were used. HCT116 was cultured using DMEM (Dulbecco's Modified Eagle Medium) (Thermo Fisher Scientific, Waltham, Massachusetts, USA), and HT29 was cultured using RPMI1640 (Loswe Parker). Memorial Institute Medium: Roswell Park Memorial Institute Medium) (Thermo Fisher Scientific). 10% FBS (Fetal Bovine Serum) (BIO-WEST Company, San Marcos, Texas, U.S.) was added to the two culture media, under 5% CO 2 , and cultured in a 37°C incubator.
[0086] 1-2. Preparation of carbonated apatite particles
[0087] 0.37g of NaHCO 3 , 90 μl of NaH 2 PO 4 2H 2 O (1M) and 180 μl of CaCl 2 (1M) was added to 100 ml of distilled water, dissolved completel...
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