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Composition for combined treatment of solid tumors

A technology of combination therapy and composition, applied in the direction of antineoplastic drugs, drug combinations, organic active ingredients, etc., can solve the problems of healthy cells and tissues with high toxic side effects, difficult drug breakthroughs, and lack of biomarkers

Active Publication Date: 2020-10-23
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Radiotherapy and chemotherapy can reach all parts of the body and can also treat some metastatic tumors. However, because radiotherapy and chemotherapy are administered systemically, they have relatively large toxic and side effects on healthy cells and tissues.
3. Antibody therapy, using antibodies to specifically block immune checkpoints related to tumor growth, such as PD-1 molecules, to initiate the body’s anti-tumor immune response process to achieve anti-tumor effects. Although this method is effective, there are The diversity of antigenic epitopes has brought about certain limitations such as low overall effectiveness and difficulty for drugs to break through the blood-brain barrier and tumor microenvironment
However, the common challenge of current targeted tumor therapy is the lack of effective biomarkers, and the evaluation of the efficacy of new targeted drugs needs to be combined with the exploration of biomarkers in future clinical trials

Method used

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  • Composition for combined treatment of solid tumors
  • Composition for combined treatment of solid tumors
  • Composition for combined treatment of solid tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 shRNA

[0025] 1. Experimental method

[0026] 1. siRNA library transfection cells

[0027] (1) Evenly spread 1.5×10 in each well of the 24-well plate 5 MC-38 cells.

[0028] (2) Prepare for transfection after 12 hours.

[0029] (3) Take 20uM Simmp3 and NC 2.5μL respectively and mix them in 100μL DMEM base culture. Take another tube and mix 2μL of Lipofectamine RNAiMAX in 100μL of DMEM medium. Vortex for 3 seconds and then stand at room temperature for 5 minutes.

[0030] (4) The above two separate reagents were mixed together by vortexing for 3 seconds and then left to stand at room temperature for 15 minutes.

[0031] (5) Add the incubated plasmid and transfection reagent and mix well.

[0032] (6) Replace with fresh complete medium after 7-12 hours;

[0033] (7) After a total of 48 hours of culture, samples can be collected to verify the expression status.

[0034] 2. Vesicular stomatitis virus VSV M51R Amplification and purification of

[0035] ...

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PUM

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Abstract

The invention discloses a composition for combined treatment of solid tumors, and the effective component at least includes an mmp3 antagonistic substance and an oncolytic virus. The composition usesthe mmp3 antagonistic substance and the oncolytic virus to perform combined treatment on the solid tumors, and can effectively inhibit tumor growth and improve survival rate.

Description

technical field [0001] The invention belongs to the technical field of cancer treatment, and in particular relates to a combined treatment composition for solid tumors. Background technique [0002] Cancer is a kind of disease that seriously endangers public health. According to statistics, in 2018, there were 18.1 million new cancer patients and 9.6 million deaths worldwide. Therefore, exploring new and effective cancer treatments is one of the current global research hotspots. [0003] At present, there are mainly the following methods for tumor treatment: 1. Surgical treatment. Surgical treatment can quickly and accurately remove tumor tissue to prevent its spread and metastasis, and is currently used more. However, the limitation of surgical treatment is that it is only useful for early-stage tumors. For advanced or metastatic tumors, the operation is difficult and the curative effect is poor. 2. Radiotherapy and chemotherapy, radiotherapy and chemotherapy is the use of...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K35/766A61K35/763A61K35/761A61K35/765A61K35/768A61K31/7105A61K31/18A61P35/00
CPCA61K45/06A61K35/766A61K35/763A61K35/761A61K35/765A61K35/768A61K31/7105A61K31/18A61P35/00A61K2300/00
Inventor 董春升熊思东胡静平梁明龙
Owner SUZHOU UNIV
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