A two-photon light field computational microscope objective
A microscope objective and two-photon technology, applied in the optical field, can solve the problems of inability to take into account simultaneously, large field of view, mutual restriction of penetration depth and high resolution index, so as to reduce the pressure of optical design and manufacturing, and improve the robustness the effect of increasing the signal-to-noise ratio of the system
Active Publication Date: 2021-06-18
ZHEJIANG UNIV +1
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[0003] The purpose of the embodiments of the present invention is to provide a two-photon light field calculation microscope objective lens to solve the problem that the existing objective lens has mutual constraints on the large field of view, penetration depth and high resolution index, and cannot be taken into account at the same time
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Embodiment 1
[0026] like figure 1 As shown, this embodiment provides a two-photon light field calculation microscope objective lens, the two-photon light field calculation microscope objective lens has an asymmetric structure between the excitation aperture and the collection aperture, adopts a sub-aperture design, and excites fluorescence with a small numerical aperture. Large numerical aperture collects energy, and greatly reduces the pressure of optical design and manufacturing while achieving near-diffraction-limited resolution; the two-photon light field calculation microscope objective lens includes a beam expander group BE and a dichroic mirror arranged in sequence along the optical axis D, water mirror O and medium W; the beam expander mirror group BE includes a focusing mirror group G1 and a collimating mirror group G2, which are used to focus the incident parallel light once and then expand the beam to collimate, and the dichroic mirror D is used In order to divide the excitation...
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Abstract
The invention discloses a two-photon light field calculation microscope objective lens, which adopts a reverse design idea to cooperate with a scanning excitation system, and includes a beam expander group BE, a dichroic mirror D, and a water mirror O arranged in sequence along the optical axis and medium W; the objective lens has an asymmetric structure between the excitation aperture and the collection aperture, and adopts a sub-aperture design to excite fluorescence with a small numerical aperture and collect energy with a large numerical aperture, which greatly reduces optical design and manufacturing while achieving near-diffraction-limited resolution. Pressure; the beam expander group BE includes a focusing lens group G1 and a collimating lens group G2, which are used to focus the incident parallel light once and then expand the beam to collimate, and the dichroic mirror D is used to combine the excitation light with the excited Fluorescence is divided into their respective optical paths. The water mirror O includes a similar beam expander group G3 and a focusing lens group G4, which expands the incident light beam and then focuses the image to improve the imaging quality. The medium W is used to increase the numerical aperture. ; According to this, the obtained objective lens has higher resolution than the traditional objective lens with the same field of view.
Description
technical field [0001] The invention belongs to the field of optics, in particular to a two-photon light field calculation microscope objective lens. Background technique [0002] Further breakthroughs in tumor treatment methods require understanding the immune processes occurring in the cellular microenvironment from the level of cell groups, which requires imaging instruments capable of high-resolution three-dimensional imaging with a large field of view. Due to the limited space bandwidth product of existing microscopes at present, the field of view and the resolution are "trade-off". Under the premise of pursuing high resolution, it is difficult to improve the field of view; in two-photon excitation, the numerical value of the objective lens can be increased The aperture achieves the purpose of improving the resolution, but at the expense of the loss of penetration depth; in addition, the traditional "excitation-imaging" shared objective mechanism requires a small numeri...
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IPC IPC(8): G02B21/02G02B21/00
CPCG02B21/0028G02B21/0072G02B21/02
Inventor 沈亦兵戴琼海胡静范静涛吴嘉敏李孙挣
Owner ZHEJIANG UNIV