Seedling stage rapid evaluation method for rape seed salt tolerance
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An evaluation method and salt tolerance technology, applied in the agricultural field, can solve the problems of large manual error, low accuracy, and high resource consumption, and achieve the effects of simple operation, accurate results and easy operation.
Inactive Publication Date: 2020-11-03
TIANJIN AGRICULTURE COLLEGE
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These methods either consume more resources or have relatively single indicators, or have low accuracy and large manual errors. Ther
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Embodiment 1
[0026] Scrub the germination box with 75% alcohol, place it for two hours, put four layers of germination paper inside; prepare a NaCl solution with a concentration of 120 mmol / L; 10 rapeseed varieties are kindly provided by the Institute of Resources and Environment, Tianjin Academy of Agricultural Sciences (available for sale ), assigned codes YC1, YC2, YC3, YC4, YC5, YC6, YC7, YC8, YC9, YC10 respectively, randomly selected plump and undamaged seeds, sterilized with 0.5% sodium hypochlorite for 15 minutes, and rinsed twice with tap water; On the germination paper of each germination box, 100 treated seeds were placed evenly at random.
[0027] The germination test was divided into two groups. One group of germination boxes was added with tap water, and the other group of germination boxes was added with 120 mmol / L NaCl solution; each treatment was repeated 4 times; the germination boxes were placed in an incubator at 20°C for the germination test. Add tap water to a group, c...
Embodiment 2
[0036] Scrub the germination box with 75% alcohol, place it for two hours, put four layers of germination paper inside; prepare a NaCl solution with a concentration of 120 mmol / L; use five rapeseed varieties YC2, YC4, YC8, YC6 and YC3, and randomly select plump, Undamaged seeds were sterilized with 0.5% sodium hypochlorite for 15 minutes, washed twice with tap water; 100 treated seeds were placed randomly and evenly on the germination paper of each germination box.
[0037] Conventional method: the germination test is divided into two groups, one group of germination boxes is added with tap water, and one group of germination boxes is added with 120mmol / L NaCl solution; each treatment is repeated 4 times; the germination box is placed in an incubator at 20°C for germination test. During the germination period, on the 2nd, 4th, and 6th days, quantitative tap water was added respectively. On the 5th day of seed germination treatment, count the germination potential of rape seed...
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Abstract
The invention relates to a seedling stage rapid evaluation method for rape seed salt tolerance. The method comprises the following steps of (1) preparing seeds; (2) preparing germination boxes; (3) preparing a NaCl aqueous solution with a corresponding salt concentration by using NaCl, adding quantitative milliliters of the prepared NaCl aqueous solution with the corresponding salt concentration on the same day in each germination box, replacing germination beds on the second, fourth and sixth days, and adding the quantitative aqueous solution with the corresponding NaCl concentration; (4) counting the germination potential on the fifth day, counting the germination rate on the seventh day, and measuring the fresh weight of seedlings on the seventh day; and calculating the relative salt damage rate of each index, and dividing oilseed rape into five salt resistance grades according to the relative salt damage rate. According to the method, the germination beds are replaced in time in the germination process, so that relatively constant salt stress is ensured; and five grade standards capable of accurately dividing the salt tolerance of the rape seeds are obtained. The method can rapidly and accurately determine the salt tolerance of the rape seeds, and can be popularized and applied in colleges, scientific research institutes and related enterprises.
Description
technical field [0001] The invention belongs to the field of agricultural technology. The invention relates to a method for rapidly evaluating the salt tolerance of rape seeds at the seedling stage. Background technique [0002] The area of salinized land in my country ranks third in the world, and the area of salinized land in Tianjin accounts for about 65.8% of the total land area of Tianjin. Soil salinization has a serious impact on the growth, quality and yield of crops. Rapeseed is widely planted throughout China and has a wide range of uses. It can be used as ornamental plants, oil crops, feed crops, green manure crops, and biofuels. [0003] Different rapeseed varieties have different ability to tolerate salt stress. Whether rapeseed can be planted on saline soil, the salt tolerance of rapeseed must first be identified and evaluated, and the salt tolerance of rapeseed varieties must be identified and evaluated, which is scientific, quick and effective The meth...
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