Three-dimensional sub-ten-nanometer positioning method and device based on polarization-modulated hollow spot illumination
A technology of polarization modulation and nano-positioning, applied in the field of super-resolution, which can solve problems such as the deterioration of dark spots and achieve the effect of avoiding negative effects
Active Publication Date: 2021-11-30
ZHEJIANG UNIV
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[0012] The above method uses two light beams to generate a three-dimensional hollow spot, which is likely to cause interference with each other, resulting in poor quality of the combined dark spot
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[0065] In the following description, many specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, therefore, the present invention is not limited to the specific embodiments disclosed below limit.
[0066] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.
[0067] Such as figure 1 As shown, the three-dimensional sub-ten nanometer positioning device of the present embodiment includes: a laser 1, a first single-mode fiber 2 and a second single-mode fiber 33, a co...
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The invention discloses a three-dimensional sub-ten-nanometer positioning method and device based on polarization-modulated hollow spot illumination. The laser light generated by the same light source is periodically modulated by an electro-optical modulator to obtain two kinds of light beams, one of which passes through 0‑2π The vortex phase plate is modulated and focused on the sample to form a transverse hollow spot, and the other beam is modulated by a 0 / π phase plate to form an axial hollow spot; the transverse hollow spot can be deflected by two electro-optic deflectors placed on the optical path, thereby Obtain the number of photons excited by the fluorescent molecules by the transverse hollow light spots at different positions, and use the electro-optic crystal zoom lens to adjust the focus of the axial hollow light spots, detect the number of photons excited by the fluorescent molecules by the axial hollow light spots at different positions, and measure the fluorescence Molecules are solved for three-dimensional space positioning. The invention adopts the polarization modulation and time-sharing method to detect the three-dimensional position of the fluorescent molecules, which can avoid the negative effect caused by the imperfection of the three-dimensional dark spots caused by the interference caused by the interference of two light beams compared with the traditional method.
Description
technical field [0001] The invention belongs to the field of super-resolution, in particular to a three-dimensional sub-ten-nanometer positioning method and device based on polarization-modulated hollow spot illumination. Background technique [0002] In 2017, Nobel Prize winner Stefan Hell published a paper [Nanometerresolution imaging and tracking of fluorescent molecules with minimal photonfluxes.Science 355(6325), 606-612(2017)] in Science, innovatively combining random coordinate positioning In order to determine the advantages of the two techniques of coordinate resolution and coordinate resolution, a new nano-resolution technique called Minimum Luminous Flux Microscopy (MINFLUX) was proposed. [0003] Different from the random coordinate positioning technology that uses Gaussian center fitting positioning, and the determined coordinate resolution technology that uses hollow beams for fluorescence loss, in the process of single molecule localization, MINFLUX only uses ...
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IPC IPC(8): G01B11/00G01N21/64G02F1/01B82Y15/00
CPCB82Y15/00G01B11/00G01N21/6402G02F1/0136
Inventor 匡翠方詹政以李传康李雨竹刘旭
Owner ZHEJIANG UNIV