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Thymosin beta4 medicinal preparation for treating depression as well as preparation method and application of thymosin beta4 medicinal preparation

A technology of pharmaceutical preparations and thymosin, which is applied in the field of new uses of peptide substances, can solve the problems of long treatment time and side effects of antidepressants

Active Publication Date: 2020-11-06
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of long treatment time and large side effects of existing antidepressant drugs, the present invention discloses a thymosin β4 pharmaceutical preparation for the treatment of depression, its preparation method and its application. Psychotic behavior induced by a stress model is reversed by doses of a pharmaceutical preparation containing thymosin β4

Method used

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  • Thymosin beta4 medicinal preparation for treating depression as well as preparation method and application of thymosin beta4 medicinal preparation
  • Thymosin beta4 medicinal preparation for treating depression as well as preparation method and application of thymosin beta4 medicinal preparation
  • Thymosin beta4 medicinal preparation for treating depression as well as preparation method and application of thymosin beta4 medicinal preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1—obtain depressed mice by chronic social frustration stress experiment;

[0034] In this example, mice with depression-like behavior were obtained by establishing a chronic social frustration stress model (CSDS). This model can simulate the depression caused by frustration in human social life, and the modeling has the advantages of strong repeatability and easy operation. . The specific model building process is as follows:

[0035] 1. The 6-week-old C57BL / 6J mice were randomly divided into experimental group and control group. For the mice in the test group, the CSDS stage lasted for 10 days and was performed at the same time period every day, such as 9:00-11:00 in the morning. C57BL / 6J mice in the test group were placed as intruders in the cage of 5-month-old male CD-1 mice, and they were physically challenged for 10 minutes to ensure the duration and effectiveness of the attack.

[0036] 2. After the frustration treatment, separate the C57BL / 6J and CD...

Embodiment 2

[0040] Embodiment 2—screening of depressed mice;

[0041] C57BL / 6J mice exposed to social defeat stress for a long time will develop a depression-like phenotype of social avoidance, which is manifested in the same period of time when socially frustrated C57BL / 6J mice are exposed to strange mice. Compared with normal C57BL / 6J mice, it will be significantly shortened. The social interaction test (social interaction test, SIT) can be used to evaluate the social avoidance behavior of mice. The test is mainly divided into the absence of strange CD1 mice (called the NoTarget stage) and the presence of strange CD1 mice. (referred to as the Target stage), and according to the ratio of the duration of entering the social contact area in the two test stages, the C57BL / 6J mice can be divided into CSDS-susceptible mice (susceptible) and CSDS-resistant mice (resilient).

[0042] 1. The social interaction test was carried out after 24 hours of CSDS treatment described in Example 1 above. ...

Embodiment 3

[0051] Embodiment 3—instill thymosin β4 through nose;

[0052] Dissolve Tβ4 in PBS to a concentration of 30, 60, and 120 μg / μL respectively. Tβ4 needs to be prepared immediately before use, and administered nasally with a 10 μL pipette.

[0053] For the selected depressed mice, they were divided into: CSDS+PBS group (instill 10 μL PBS, 5 μL in each nostril), CSDS+12 mg / kg Tβ4 group (instill 10 μL with a concentration of 30 μg / μLTβ4, 5 μL in each nostril); CSDS+ The 24mg / kg Tβ4 group was instilled with 10 μL of 60 μg / μL Tβ4, 5 μL in each nostril; the CSDS+48 mg / kg Tβ4 group was instilled with 10 μL of 120 μg / μL Tβ4, 5 μL in each nostril.

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Abstract

The invention discloses a thymosin beta4 medicinal preparation for treating depression as well as a preparation method and application of the thymosin beta4 medicinal preparation, and belongs to the technical field of disease treatment. The mouse depressive behavior is induced mainly by establishing a chronic social defeat stress model (CSDS), and the model has the advantages that the model simulates social attributes in human chronic stress, and then after nasal administration of T beta 4 is conducted for seven consecutive days, the mouse depressive behavior is induced. Through a social interaction test (SIT), a tail suspension test (TST), a sucrose preference test (SPT) are and other behavioral tests are carried out to evaluate the depression level, and the result shows that T beta4 hasa rapid anti-depression effect in mice, and can reverse CSDS-induced depression-like behaviors within 7 days.

Description

technical field [0001] The invention relates to a new application of a peptide substance, which belongs to the technical field of disease treatment, and in particular relates to a thymosin β4 pharmaceutical preparation for treating depression, a preparation method and an application thereof. Background technique [0002] The main indications of mental illness are obvious and persistent abnormalities in cognition, emotion, volition and behavior of patients, which are difficult to be accepted by social routines, severely impaired social and mental functions, and cannot maintain normal study, work and life. Aggressive behavior, self-mutilation, or suicide can occur under the dominance of pathological cognitions and emotions. Mental illness has become a major medical and social problem in today's society. [0003] Clinically, drugs are the main means and methods for the treatment of mental illness. Drugs for the treatment of mental illness mainly include antipsychotics, antide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/22A61P25/24A01K67/027A61B5/16
CPCA01K67/027A01K2207/20A01K2227/105A01K2267/0356A61B5/165A61B2503/40A61B2503/42A61K38/2292A61P25/24
Inventor 丁一丁明星刘爱杨迪琦尹瑞玲南沙张岩岩雷蔷会
Owner HUAZHONG AGRI UNIV
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