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Sample classification device for gene marker detection

A gene marker and classification device technology, which is applied in the field of sample classification devices for gene marker detection, can solve the problems of low blood cell recovery rate, aspiration, inability to separate accurately, and achieves avoiding mutual contamination, simple use and operation, and simple structure. Effect

Pending Publication Date: 2020-11-13
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the blood is usually centrifuged, and then the operator performs the suction operation, which is easy to cause aspiration, and there are also problems such as relatively low recovery rate of blood cells and inability to separate accurately.

Method used

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  • Sample classification device for gene marker detection

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Experimental program
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Effect test

Embodiment 1

[0036] A sample sorting device for gene marker detection, such as figure 1 As shown, it includes a centrifuge tube 1, a piston 11 is connected to the bottom of the centrifuge tube 1, and three collection tubes 2 are connected in parallel at the side end of the centrifuge tube 1, and the three collection tubes 2 communicate with the centrifuge tube 1 through a draft tube 3.

[0037] The bottom surface of the centrifuge tube 1 is a circular hollow cylindrical centrifuge tube 1, the top surface is rotatably connected with a top cover 13, the bottom is sealed with a rubber piston 11, and the bottom of the piston 11 is connected with a sealed bottom 12. The thickness of the tube wall at the joint between the sealing bottom 12 and the bottom of the centrifuge tube 1 is thinned, and the sealing bottom 12 can be folded off from the bottom of the centrifuge tube 1 under the action of an external force, so that the piston 11 can be used conveniently.

[0038] One side of the centrifuge ...

Embodiment 2

[0041] A method of using the sample classification device for gene marker detection of embodiment 1, comprising the following steps:

[0042] (1) Open the top cover 13, put the blood sample into the centrifuge tube 1, place it in a centrifuge for centrifugation, and the blood is layered, the top is the plasma layer 4, the middle is the blood cell layer 5, and the bottom red blood cell layer 6 ;

[0043](2) Break off the sealing bottom 12 at the bottom of the centrifuge tube 1 and take it off, and insert the hollow needle 31 into the centrifuge tube 1 above the liquid level of the sample;

[0044] (3) Push the piston 11 to make the liquid level in the centrifuge tube 1 rise, turn off the switch one 321 and the switch two 322, open the switch three 323, so that the uppermost plasma layer 4 flows into the third collection tube 232;

[0045] (4) After observing that the plasma layer 4 almost completely flows out of the centrifuge tube 1, turn off the switch three 323 and turn on ...

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Abstract

The invention provides a sample classification device for gene marker detection and a using method thereof, and relates to the technical field of gene diagnosis. The sample classification device for gene marker detection comprises a sealed centrifugal tube with a top cover, the centrifugal tube is connected with at least one collecting tube in parallel, the collecting tubes are detachably connected with the centrifugal tube, and the bottom of the centrifugal tube is connected with a driving piece for driving the bottom to move up and down. The device is simple in structure, different components in blood are effectively separated and recycled, especially blood cells are collected, and genomic DNA is convenient to extract.

Description

technical field [0001] The invention relates to the technical field of gene diagnosis, in particular to a sample classification device for gene marker detection and a use method thereof. Background technique [0002] In recent years, with the development of molecular biology, biomarkers have become an important class of substances for clinical evaluation of diseases or injuries. They can not only explore the pathogenesis of diseases at the molecular level, but also accurately and sensitively evaluate early tissue damage. And it has its unique advantages to provide early warning and provide a good basis for auxiliary diagnosis in clinic. Among them, gene markers are one of the biomarkers, which can be used to monitor the abnormal expression of some genes, and can be used to diagnose or warn of certain diseases related to abnormal gene expression. [0003] For the detection of gene markers, it is often necessary to extract the patient's genomic DNA first to perform correspond...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12Q1/6806
CPCC12Q1/6806C12Q2523/32
Inventor 孙杰周猛
Owner WENZHOU MEDICAL UNIV
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