POCT-based fluorescent quantitative PCR instrument calibration method and PCR instrument

Abstract

The invention relates to the technical field of PCR detection devices, and provides a POCT-based fluorescent quantitative PCR instrument calibration method and a PCR instrument. The PCR instrument comprises a test tube rack, a reagent tube, a light source module and an acquisition module; the calibration method comprises the following steps: marking a hole site with a reagent tube as at least onereference hole and a plurality of test holes; driving the light source module to emit exciting light; acquiring an image through the acquisition module, and extracting a gray value of the image; dividing the gray value of the extracted image into a reference hole gray value and a test hole gray value according to the reference hole and the test hole; calculating a calibration coefficient of each test hole according to the reference hole gray value of the reference hole and the test hole gray value of each test hole; and calculating to obtain the calibrated test hole gray value of each test hole according to the test hole gray value of each test hole and the calibration coefficient of each test hole. The problem of fluorescence deviation of each hole site caused by different included anglesbetween the reagent tube and the optical axis of the lens is overcome, and the gene detection accuracy is ensured.

Description

technical field [0001] The invention relates to the technical field of PCR detection devices, in particular to a method for calibrating a POCT-based fluorescence quantitative PCR instrument and a PCR instrument. Background technique [0002] The traditional fluorescent quantitative PCR instrument is designed according to the requirements of scientific research application scenarios. The reaction tube adopts a general-purpose eight-tube tube. The fluorescence detection system is set above the reaction tube. The excitation light source is injected from the top of the reagent tube. The top of the tube is detected, so the fluorescence detection system needs to be equipped with a hot cover. The hot cover and the reaction tube cover are made of highly transparent optical materials. Components, the entire optical path is relatively complicated, the volume is large, the cost is expensive, and the production and adjustment process is complicated. [0003] In order to improve the det...

AI Technical Summary

Problems solved by technology

For this reason, the first aspect of the present invention proposes a method for calibrating a fluorescent quantitative PCR instrument based on POCT, so that the performance of a fluorescent quantitative PCR instrument based on POCT can meet the needs of clinical applications; SNP gene detection has outstanding problems such as complex operation, long inspection cycle, and high detection environment (must meet the requirements of PCR laboratory)

Images