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Hepatocellular carcinoma PDX model construction method

A technology of hepatocellular carcinoma and its construction method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problems of low modeling success rate and high tumor formation rate of hepatocellular carcinoma PDX model

Active Publication Date: 2020-11-17
ZHEJIANG UNIV
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Problems solved by technology

[0013] The present invention aims at the problem of low modeling success rate of the PDX model of hepatocellular carcinoma in the prior art, and provides a composition for judging the tumor formation rate when the PDX model of hepatocellular carcinoma is constructed for the purpose of non-medical diagnosis, and A method for constructing a PDX model of hepatocellular carcinoma. By predicting the tumor formation rate of the PDX model of hepatocellular carcinoma, samples with a high tumor formation rate are selected for modeling, and samples with a low tumor formation rate or with no possibility of tumor formation can be discarded modeling

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  • Hepatocellular carcinoma PDX model construction method
  • Hepatocellular carcinoma PDX model construction method
  • Hepatocellular carcinoma PDX model construction method

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Embodiment 1

[0041] Liver cancer PDX modeling and tumor formation prediction analysis method:

[0042] Patients signed the informed consent form before the operation;

[0043] a. Acquisition of tumor tissue: fresh tumor tissue (coded as F0) from patients with hepatocellular carcinoma was obtained under aseptic conditions in the operating room, placed in DMEM / F12 medium, and transported to the animal room on ice within 2 hours;

[0044]b. Divide the tumor tissue into three parts in the ultra-clean bench: the first part is to cut the tumor tissue block into small pieces with a diameter of about 2 mm in a sterile environment, and quickly plant them into the immune-deficient mice; the second part is to The tumor tissue blocks were fixed in formalin solution and embedded in paraffin for later molecular pathological detection; the third part was to immediately freeze the tumor tissue blocks with liquid nitrogen for DNA / RNA extraction;

[0045] c. Construction of PDX model: The small pieces of t...

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Abstract

Disclosed are: a composition for determining the tumor formation rate during the construction of a hepatocellular carcinoma PDX model, which is intended for non-disease treatment; and a hepatocellularcarcinoma PDX model construction method using the composition. The composition for judging the tumor formation rate during the construction of the hepatocellular carcinoma PDX model comprises GPC3 and Ki67 in a hepatocellular carcinoma tumor tissue sample, and the tumor formation rate of PDX modeling can be judged by detecting the two proteins. According to the method for predicting tumorigenesisof the hepatocellular carcinoma PDX model, the immunohistochemical results of tumor tissues GPC3 and Ki67 of a hepatocellular carcinoma patient are interpreted, and whether tumorigenesis of the PDX model occurs or not is predicted in an early stage. The PDX model is predicted not to be nodulated in the early stage, and the PDX model can be constructed as soon as possible.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for constructing a PDX model of hepatocellular carcinoma. Background technique [0002] PDX (Patient-Derived Xenograft) patient-derived tumor xenograft model refers to planting fresh tumor tissue from patients into immunodeficient mice to achieve growth. The PDX model is characterized by retaining the morphological and structural features, genetic features, and molecular features of the patient's tumor to the greatest extent, which can provide an important platform for studying tumor biology, finding markers, and screening drug targets. PDX models can accurately reflect the efficacy of anti-tumor drugs, and provide important medication suggestions and guidance for individualized precision treatment of clinical cancer patients. my country is a country with a high incidence of liver cancer, of which 90% of liver cancer is hepatocellular carcinoma, which ranks first in the mal...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574A01K67/027
CPCG01N33/57484G01N33/57438A01K67/0271A01K2207/12A01K2227/105A01K2267/0331
Inventor 卓建勇鲁迪王建国连正星张佳丽李慧慧岑贝妮徐骁
Owner ZHEJIANG UNIV
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