Preparation method and application of lactoferrin peptide enzymatic hydrolysate with antibacterial activity

A technology of lactoferrin and peptide enzymatic hydrolyzate, which is applied in the field of preparation of lactoferrin peptide enzymatic hydrolyzate, can solve the problem of limited enzymatic hydrolysis efficiency of lactoferrin active peptide enzymatic hydrolyzate, large amount of pepsin used, high preparation cost, etc. problem, achieve the effect of reducing preparation time, reducing preparation cost, and strong antibacterial activity

Pending Publication Date: 2020-11-20
镇江市天益生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The problems that lead to the current commonly used technology are: (1) the use of pepsin is relatively large, and the preparation cost is high; (2) the enzymatic hydrolysis preparation takes a long time; (3) the obtained lactoferrin active peptide is enzymatic Liquid enzymatic hydrolysis has limited efficiency and low activity

Method used

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  • Preparation method and application of lactoferrin peptide enzymatic hydrolysate with antibacterial activity

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Experimental program
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Effect test

Embodiment 11

[0020] Preparation by the method of the present invention: mix lactoferrin and deionized water (1:15) to prepare lactoferrin aqueous solution; use HCl solution to adjust the pH of the system to 2.3 to obtain lactoferrin acidic solution; The ferritin acidic solution was subjected to steam explosion treatment with a pressure of 0.4MPa and a holding time of 0.5min; then the above-mentioned lactoferrin acidic solution was subjected to ultrasonic treatment with an ultrasonic power of 180W and an ultrasonic time of 2s; pepsin (20U / 10mL) Add it to the above lactoferrin solution, put it in a water bath at 37°C for 5 minutes, then cool it to 4°C; add NaOH solution to adjust the pH of the system to 7, then centrifuge at 3000rpm to obtain the supernatant, and obtain the lactoferrin active peptide hydrolyzate A1 .

Embodiment 12

[0022] Preparation by the method of the present invention: mix lactoferrin and deionized water (1:5) to prepare lactoferrin aqueous solution; use HCl solution to adjust the pH of the system to 1.2 to obtain lactoferrin acidic solution; The ferritin acidic solution was subjected to steam explosion treatment with a pressure of 1.0MPa and a holding time of 0.2min; then the above-mentioned lactoferrin acidic solution was subjected to ultrasonic treatment with an ultrasonic power of 300W and an ultrasonic time of 6s; pepsin (30U / 10mL) Add it to the above lactoferrin solution, put it in a water bath at 40°C for 10 minutes, then cool to 2°C; add NaOH solution to adjust the pH of the system to 6.5, and then centrifuge at 2500rpm to obtain the supernatant to obtain lactoferrin active peptide hydrolyzate A2 .

Embodiment 13

[0024] Preparation by the method of the present invention: mix lactoferrin and deionized water (1:30) to prepare lactoferrin aqueous solution; use HCl solution to adjust the pH of the system to 3 to obtain lactoferrin acidic solution; The ferritin acidic solution was subjected to steam explosion treatment with a pressure of 0.2 MPa and a holding time of 3 min; then the above-mentioned lactoferrin acidic solution was subjected to ultrasonic treatment with an ultrasonic power of 150 W and an ultrasonic time of 4 s; adding pepsin (3 U / 10 mL) Put it into the above lactoferrin solution, put it in a water bath at 35°C for 8 minutes, then cool to 6°C; add NaOH solution to adjust the pH of the system to 7, and then centrifuge at 1000rpm to obtain the supernatant to obtain lactoferrin active peptide hydrolyzate A3.

[0025] control group

[0026] Mix lactoferrin and deionized water (1:15) to prepare a lactoferrin aqueous solution; use HCl solution to adjust the pH of the system to 2.3 ...

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Abstract

The invention provides a preparation method of lactoferrin peptide enzymatic hydrolysate with antibacterial activity. The preparation method comprises the following specific steps: preparing a lactoferrin water solution, and obtaining a lactoferrin acidic solution under a certain pH value in an acid system; sequentially carrying out steam explosion treatment and ultrasonic treatment on the lactoferrin acid solution; adding pepsase with a certain concentration into the solution treated in the step 3, carrying out constant-temperature water bath for a period of time, slowly cooling to a certaintemperature, adding into an alkaline system, and carrying out centrifugal treatment, so as to obtain supernatant, namely lactoferrin peptide enzymatic hydrolysate. According to the method, the problems that in the process of preparing the lactoferrin peptide enzymatic hydrolysate from pepsase, the use amount of the pepsase is relatively large, the preparation cost is high, the preparation period is relatively long, and the obtained lactoferrin active peptide enzymatic hydrolysate is low in enzymolysis efficiency and low in antibacterial activity can be solved.

Description

technical field [0001] The invention belongs to the technical field of food additives and food nutrition enhancers, in particular to a preparation method and application of a lactoferrin peptide enzymatic hydrolyzate with antibacterial activity. Background technique [0002] At present, lactoferrin peptides are often prepared by adding pepsin to lactoferrin solution, but considering that pepsin hydrolyzes protein substrates depends on substrate concentration, substrate characteristics, pH value, temperature, reaction time and other conditions. The mass concentration of substrate and the mass concentration ratio of enzyme and substrate are the two most important factors in the enzymatic hydrolysis system. Especially for site-specific catalysts such as proteases, not only substrate inhibition occurs, that is, the enzymolysis reaction conforms to the Mie equation at low substrate concentrations, but when the substrate concentration increases, the reaction rate decreases instead...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/34A23L33/18
CPCA23J3/343A23L33/18A23V2002/00A23V2250/55A23V2300/38
Inventor 李珮铷魏雪倩陶旭
Owner 镇江市天益生物科技有限公司
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