Capsular polysaccharide vaccine protein nucleic acid removal device and removal process

Abstract

The invention discloses a capsular polysaccharide vaccine protein nucleic acid removal device and removal process. The capsular polysaccharide vaccine protein nucleic acid removal device comprises a base, an inclination adjuster, a flowing groove box, a buffer solution inlet, a protein injection port, an injection funnel, a positive plate, a negative plate, a nucleic acid discharge pipe, a proteindischarge pipe, a buffer solution storage box, a liquid suction pump, a liquid adding port, a voltage adapter, a wire and a bearing, wherein the flowing groove box is cooperatively connected to the outer wall of one side of the top end of the base through the inclination adjuster. The capsular polysaccharide vaccine protein nucleic acid removal device is relatively compact in structure and relatively high in automation level; the inclination angle of the flowing groove box can be adjusted through the inclination adjuster, so that the flow speed of an internal buffer solution is adjusted and controlled, later-period electrophoretic separation and removal of vaccine protein nucleic acid are facilitated, and voltage control during electrophoresis can be performed; and in the capsular polysaccharide vaccine protein nucleic acid removal process, the flow rate of the buffer solution is standardized.

Description

Technical field [0001] The invention relates to the technical field of protein nucleic acid removal, in particular to a capsular polysaccharide vaccine protein nucleic acid removal device and removal process. Background technique [0002] There are a variety of carbohydrates in bacteria, which play an important role in bacterial recognition, signal transmission, adhesion, infection and defense, among which pathogenicity is related to CPS and lipopolysaccharide LPS in the O antigen polysaccharide O- PS etc. Due to the immunogenicity of polysaccharides, vaccines made from specific polysaccharides can be called polysaccharide vaccines after purification. Capsular polysaccharide vaccines often need to separate and remove proteins and nucleic acids during the production process. Electrophoresis is often used for separation and removal. These methods usually require more complicated manual operations or the help of a variety of instruments and equipment. The operation is also insuffic...

AI Technical Summary

Problems solved by technology

Due to the immunogenicity of polysaccharides, specific polysaccharides can be purified and made into vaccines called polysaccharide vaccines. In the production process of capsular polysaccharide vaccines, proteins and nucleic acids often need to be separated and removed. Proteins and nucleic acids are on the market Electrophoresis is often used for the separation and removal of capsular polysaccharide vaccines. These methods usually require more complicated manual operations or with the help of a variety of instruments and equipment. The operation is also insufficient in time and removal efficiency. Therefore, a convenient method for removing capsular polysaccharide vaccine protein nucleic acids The device is very necessary. The process of removing capsular polysaccharide vaccine protein and nucleic acid has not been perfected in the market, and the buffer flow rate cannot be adjusted. Among them, the buffer flow rate has not been regulated, which affects the removal of capsular polysaccharide vaccine protein and nucleic acid. craft

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