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System for extracting biomolecules from sample and related methods

A technology of sample tubes and automation systems, applied in biological material sampling methods, biochemical equipment and methods, specific-purpose bioreactors/fermenters, etc., which can solve problems such as limitations, poor research results, and inaccurate diagnostic determinations

Pending Publication Date: 2020-11-24
NANJING GENSCRIPT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Despite several benefits of known automated systems for isolating biomolecules from biological samples, such systems often suffer from incomplete biomolecule recovery and cross-contamination, have limited sample processing throughput, and are often limited to processing very small sample volume
These shortcomings may lead to inaccurate diagnostic assays or poor study results

Method used

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  • System for extracting biomolecules from sample and related methods
  • System for extracting biomolecules from sample and related methods
  • System for extracting biomolecules from sample and related methods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0147] Embodiment 1. A liquid handling system comprising:

[0148] At least one pipette system comprising:

[0149] A two-channel pipette comprising an upper region attached to a support structure, and a lower dispensing region comprising a fluid connection on the side of the dispensing region to a first channel a first liquid port, and a second liquid port at the tip of the dispensing area fluidly connected to the second channel;

[0150] a control valve that controls the flow of liquid through the first channel or the second channel of the pipette; and

[0151] a pump that is fluidly connected to the control valve.

Embodiment approach 2

[0152] Embodiment 2. The liquid handling system of embodiment 1, wherein the second channel of the dual channel pipette passes through and is parallel to the first channel of the dual channel pipette.

Embodiment approach 3

[0153]Embodiment 3. The liquid handling system of embodiment 1, wherein the second channel of the dual channel pipette is adjacent to the first channel of the dual channel pipette.

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Abstract

An automated system for isolating biomolecules from a biological sample is described herein. Further described are methods for operating such systems. Also described are components of the automated system, such as a liquid handling system, a robotic arm, sample tube racks, an analytical instrument, a barcode reader, and sample processing modules, which can include a shaker, a magnetic bead biomolecule isolation system, an endotoxin control system, a heated incubator, or a chilled incubator.

Description

[0001] Cross References to Related Applications [0002] This application claims the priority and benefit of the international patent application PCT / CN2018 / 083155 filed on April 16, 2018, the disclosure of which is incorporated herein by reference in its entirety. technical field [0003] The present invention relates to automated sample processing systems and components thereof, including liquid handling systems, sample tube holders and magnetic bead separation systems adapted for use with the liquid handling systems, and methods of use thereof. Background technique [0004] Automated systems have previously been used to isolate nucleic acids from biological samples. Such systems allow for increased efficiency and quality control compared to manual benchtop separation techniques. from QIAGEN under the trade name The automated system sold by SP Systems is an exemplary system for automated processing of biological samples for nucleic acid isolation. Such automated syste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/26B01L9/00B01L9/06
CPCB01L9/06B01L3/0217G01N35/1065G01N35/0098G01N2035/00346G01N35/1004
Inventor 朱金鑫贺瑞娜钱红白涛望超李德铭陈国栋
Owner NANJING GENSCRIPT BIOTECH CO LTD
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