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Bifidobacterium breve and application thereof

A bifidobacterium breve and drug technology, applied in the field of microorganisms, can solve the problems of unsuitable long-term application and discomfort for patients with chronic constipation, and achieve the effects of protecting the liver, protecting the intestinal mucosa, and alleviating obesity

Active Publication Date: 2020-11-27
深圳市创新医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many drugs available for the treatment of constipation, but most of them are not suitable for patients with chronic constipation, nor are they suitable for long-term use

Method used

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  • Bifidobacterium breve and application thereof
  • Bifidobacterium breve and application thereof
  • Bifidobacterium breve and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation, purification and identification of bacterial strains

[0029] 1. Strains, medium and culture conditions

[0030] (1) Bacterial source

[0031] A new Bifidobacterium breve was obtained through strain isolation and screening in a centenarian.

[0032] The host’s health status and dietary characteristics: The centenarian was able to move freely, be in a good mental state, and have a normal body mass index. The daily diet is mainly grains and white meat, and about 50ml of white wine is drunk every day.

[0033] (2) Medium

[0034] Enrichment medium: 5g / L yeast powder, 10g / L peptone and 2.5g / L Nacl;

[0035] Plate medium: 20g / L glucose, 2g / L (NH 4 ) 2 SO 4 , 13.3g / L KH 2 PO 4 , 1.2g / L MgSO 4 . 7H 2 O, 1.7g / L citric acid, 1.7g / L trace element solution, 15g / L agar, 0.5μg / mL Nile blue A.

[0036] 2. Isolation and identification of strains

[0037] (1) Isolation of strains

[0038] Take about 0.2 g of the sample, add 1 ml of MRS medium, vortex...

Embodiment 2

[0049] Example 2 The Relief Effect and Mechanism of Lactobacillus breve TR103 on Intestinal Mucosa Injury in Mice

[0050] 1. Establishment and grouping of acute ulcerative colitis mouse model

[0051] Sixty male BALB / c mice were randomly divided into 5 groups: normal group, model group and TR103 low, medium and high dose groups, 12 in each group.

[0052] From the first day of the experiment, the normal group and the model group were administered with sterile normal saline. 6 , 1×10 8 , 1×10 10 The cfu / ml TR103 bacterial solution was orally administered to each mouse once a day, 0.2 mL each time, for a total of 14 days.

[0053] From the 8th day of the experiment, referring to the method of Murthy et al., the distilled water drunk by the mice in the model group and TR103 low, medium, and high dose groups was changed to 2.5% DSS solution, and they drank freely for 7 days to form an experimental colitis model. During the period, the body weight, stool properties and occult ...

Embodiment 3

[0060] Example 3 Research on the effect of Lactobacillus breve TR103 on alleviating chronic alcoholic liver injury in mice

[0061] 1. Grouping and handling of experimental animals

[0062] 5 ICR mice were raised in a cage, free to drink water and eat, change the litter regularly, keep the cage clean and dry, and after adaptive feeding for 1 week, they were randomly divided into 4 groups with 15 mice in each group; , alcohol injury group, Lactobacillus breve TR103 low-dose test group (1.0×10 8 CFU / mL), Bifidobacterium breve TR103 high-dose test group (1.0×10 10 CFU / mL).

[0063] From 0 to 2 weeks after the start of the experiment, mice in the control group and alcohol injury group were given intragastric administration of normal saline 0.2mL / (mouse·d), and mice in each group of Bifidobacterium breve TR103 were orally administered 0.2mL / (mouse·d). d). From the third week, on the basis of the gavage method in the previous two weeks, after an interval of half an hour, the con...

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Abstract

The invention also provides Bifidobacterium breve TR103 which is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M2018728; the preservation address is Wuhan University in Wuhan, Hubei province of the People's Republic of China; and the preservation date is October 31, 2018. The bifidobacterium breve TR103 provided by the invention is derived from theelderly with long life, and has the effects of protecting intestinal mucosa, protecting liver, avoiding liver injury, regulating defecation frequency and relieving obesity.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a bifidobacterium breve, and also relates to the application of the strain. Background technique [0002] The main factors causing intestinal mucosal damage are severe trauma, severe infection, burns, shock, acute pancreatitis and other critically ill patients may have damage to the intestinal mucosal barrier, and the latter can aggravate the conditions of various primary diseases. Promote, reciprocal cause and effect. Among them, chemotherapy drugs, abdominal dialysis, bacterial peritonitis, inflammatory bowel disease and other diseases are the most common damage to intestinal mucosa, and intestinal mucosal barrier damage will cause bacterial translocation, so that pathogenic bacteria parasitic in the large intestine can be translocated to the small intestine ( Transverse displacement), can also cross the intestinal mucosa into the mesenteric lymph nodes and portal vein, and then ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P1/00A61P1/16A61P1/10A23L33/135C12R1/01
CPCC12N1/20A61K35/745A61P1/00A61P1/16A61P1/10A23L33/135A23V2002/00C12R2001/01C12N1/205A23V2400/519A23V2200/3204A23V2200/334A23V2200/332
Inventor 李珮铷贾长生边高瑞王信超李苗许登泉王林
Owner 深圳市创新医学科技有限公司
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