Bifidobacterium breve and application thereof
A bifidobacterium breve and drug technology, applied in the field of microorganisms, can solve the problems of unsuitable long-term application and discomfort for patients with chronic constipation, and achieve the effects of protecting the liver, protecting the intestinal mucosa, and alleviating obesity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Example 1 Isolation, purification and identification of bacterial strains
[0029] 1. Strains, medium and culture conditions
[0030] (1) Bacterial source
[0031] A new Bifidobacterium breve was obtained through strain isolation and screening in a centenarian.
[0032] The host’s health status and dietary characteristics: The centenarian was able to move freely, be in a good mental state, and have a normal body mass index. The daily diet is mainly grains and white meat, and about 50ml of white wine is drunk every day.
[0033] (2) Medium
[0034] Enrichment medium: 5g / L yeast powder, 10g / L peptone and 2.5g / L Nacl;
[0035] Plate medium: 20g / L glucose, 2g / L (NH 4 ) 2 SO 4 , 13.3g / L KH 2 PO 4 , 1.2g / L MgSO 4 . 7H 2 O, 1.7g / L citric acid, 1.7g / L trace element solution, 15g / L agar, 0.5μg / mL Nile blue A.
[0036] 2. Isolation and identification of strains
[0037] (1) Isolation of strains
[0038] Take about 0.2 g of the sample, add 1 ml of MRS medium, vortex...
Embodiment 2
[0049] Example 2 The Relief Effect and Mechanism of Lactobacillus breve TR103 on Intestinal Mucosa Injury in Mice
[0050] 1. Establishment and grouping of acute ulcerative colitis mouse model
[0051] Sixty male BALB / c mice were randomly divided into 5 groups: normal group, model group and TR103 low, medium and high dose groups, 12 in each group.
[0052] From the first day of the experiment, the normal group and the model group were administered with sterile normal saline. 6 , 1×10 8 , 1×10 10 The cfu / ml TR103 bacterial solution was orally administered to each mouse once a day, 0.2 mL each time, for a total of 14 days.
[0053] From the 8th day of the experiment, referring to the method of Murthy et al., the distilled water drunk by the mice in the model group and TR103 low, medium, and high dose groups was changed to 2.5% DSS solution, and they drank freely for 7 days to form an experimental colitis model. During the period, the body weight, stool properties and occult ...
Embodiment 3
[0060] Example 3 Research on the effect of Lactobacillus breve TR103 on alleviating chronic alcoholic liver injury in mice
[0061] 1. Grouping and handling of experimental animals
[0062] 5 ICR mice were raised in a cage, free to drink water and eat, change the litter regularly, keep the cage clean and dry, and after adaptive feeding for 1 week, they were randomly divided into 4 groups with 15 mice in each group; , alcohol injury group, Lactobacillus breve TR103 low-dose test group (1.0×10 8 CFU / mL), Bifidobacterium breve TR103 high-dose test group (1.0×10 10 CFU / mL).
[0063] From 0 to 2 weeks after the start of the experiment, mice in the control group and alcohol injury group were given intragastric administration of normal saline 0.2mL / (mouse·d), and mice in each group of Bifidobacterium breve TR103 were orally administered 0.2mL / (mouse·d). d). From the third week, on the basis of the gavage method in the previous two weeks, after an interval of half an hour, the con...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com