Forward regulation factor for regulating included angle of corn leaves and application thereof

A corn leaf angle, positive regulation technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problem of insufficient number of genes

Active Publication Date: 2020-12-08
HENAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the identified QTL, the number of identified genes related to leaf angle is far from en...

Method used

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  • Forward regulation factor for regulating included angle of corn leaves and application thereof
  • Forward regulation factor for regulating included angle of corn leaves and application thereof
  • Forward regulation factor for regulating included angle of corn leaves and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Construction of near isogenic lines

[0029] The maize inbred line Yu 537A with small leaf angle and compact plant type was used as the donor parent, and the maize inbred line Shen 137 with large leaf angle and loose plant type was used as the recipient parent through backcrossing combined with molecular marker-assisted selection technology to construct near-isogenic lines ( figure 1 ).

[0030] In the spring of 2014, the combination of two parents in Zhengzhou won F 1 ; F planted in Hainan in the winter of 2014 1 Progeny and recipient parent Shen 137, with Shen 137 and F 1 Backcross to get BC 1 f 1 ;

[0031] According to BC 1 f 1 The phenotype selection of 13 plants with small leaf angles was planted in Zhengzhou in the spring of 2015 to form ear rows, and continued to backcross with Shen 137 to obtain BC 2 f 1 ; Fifteen individual plants with small leaf angles were selected from them, and they were planted in Sanya, Hainan in the winter of 2015, and then b...

Embodiment 2

[0035] Separation by map-based cloning ZmCLA7-1 Gene

[0036] Use BC 4 f 2 The population of 8723 strains was used as a fine-positioning population, and the single strain 812 with a leaf angle less than or equal to 20° was selected from it. qLA7-1 Two-sided markers PZE-07122629825 and PZE-107077981 were used to analyze their genotypes, and 12 individual plants with recombination and exchange at PZE-07122629825 or PZE-107077981 were selected; at the same time, 673 individual plants with leaf angles greater than 25° were selected. Combined with molecular marker selection, 7 exchanged individual plants with large leaf angles were screened out. Using newly developed and highly polymorphic 12 pairs of primers SSR7-1, SSR7-2, SSR7-3, SSR7-4, SSR7-5, SSR7-6, SSR7-8, SSR7-9, SSR7-10, SSR7 -11. SSR7-12 (Refer to Zhang Jun for the marker development method, Map-based cloning and functional analysis of the ZmCLA4 gene related to the angle between maize leaves, Henan Agricultural Uni...

Embodiment 3

[0040] ZmCLA7-1 Positive regulation of corn leaf angle

[0041] ZmCLA7-1 The leaf angle was increased in Shen 137, and the leaf angle was decreased in Shen 137-NIL76, so we detected the expression of ZmCLA7-1 gene. qRT-PCR detection results showed that the leaves from the 6-12 leaf stage of maize growth and development ( image 3 ) efficient expression. For different materials, ZmCLA7-1 The expression level in Shen 137 at different periods was significantly higher than Shen 137-NIL76 ( image 3 ),show ZmCLA7-1 The leaf angle of maize with high expression level is relatively smaller, thus it is proposed that ZmCLA7-1 Regulates the angle between maize leaves as a positive regulator.

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Abstract

The invention belongs to the field of crop genetic breeding, and relates to a ZmCLA7-1 gene for regulating the included angle of corn leaves, in particular to a forward regulation factor for regulating and controlling the included angle of corn leaves and application thereof. Wherein the forward regulation factor is the gene ZmCLA7-1 or a promoter of the gene ZmCLA7-1, the base sequence of the gene ZmCLA7-1 is as shown in SEQ ID NO.1, the promoter sequence is as shown in SEQ ID NO.5, and the promoter of the gene ZmCLA7-1 has base mutation and insertion/deletion. RNAi technology is used for inhabiting the expression of endogenous ZmCLA7-1 gene of corn. Specifically, the ZmCLA7-1 gene is fused with other regulatory elements such as a constitutive promoter (such as a CaMV35S promoter) or an organ specific promoter to construct a gene inhibition expression vector, and a corn selfing line with a small leaf included angle and a compact plant type is created through a transgenic technology (such as antisense RNA or RNAi) and is used for cultivating a new corn density-tolerant variety.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a method for regulating the included angle of corn leaves. ZmCLA7-1 The gene, in particular, refers to a positive regulatory factor for regulating the included angle of maize leaves and its application. Background technique [0002] Food security is a major issue related to the national economy and people's livelihood. Irreversible factors such as the continuous decrease of cultivated land, continued population growth, and continuous increase in food demand determine that food security is a long-term and arduous task to ensure the stable development of my country's society and economy. Therefore, achieving a further breakthrough in the level of per unit yield on the current level of production is the core of realizing the task of increasing my country's grain production. Practice at home and abroad has proved that increasing the density of corn planting is an important t...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/113C07K14/415C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8218C12N15/8261
Inventor 陈彦惠库丽霞任真真胡冠军
Owner HENAN AGRICULTURAL UNIVERSITY
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