In-situ chemical fixing device for deep-sea biological sample

Abstract

The invention discloses an in-situ chemical fixing device for a deep-sea biological sample. The in-situ chemical fixing device comprises a bottle body, a clamping cover, a releaser unit and a fixing frame; the two ends of the bottle body are open, the clamping covers are located at the two openings of the bottle body, the two ends of the rubber rope located in the bottle body are connected with the inner sides of the two clamping covers respectively, and the outer sides of the clamping covers are connected through the releaser unit. Two fixing frames are arranged in the bottle body, and a mother liquor bag is arranged between the two fixing frames; bayonet limiting holes are formed in the fixing frames, bayonet bodies are arranged in the bayonet limiting holes of the two fixing frames, thesharp ends of the bayonet bodies are opposite to the mother liquor bag, and the blunt ends of the bayonet bodies extend to the opening of the bottle body. The device can be widely applied to variousapplication scenes including application in a high-pressure simulation bin, is simple in structure and convenient to use, and improves the efficiency of chemical fixation of biological samples.

Description

technical field [0001] The invention belongs to the technical field of deep-sea sampling equipment, and in particular relates to an in-situ chemical fixation device for deep-sea biological samples, which can be applied to the collection and chemical fixation of biological samples in different water bodies. Background technique [0002] With the gradual refinement of life science exploration, it is usually necessary to detect the gene expression pattern or chromatin three-dimensional configuration information presented by biological samples in the original environment, which requires in situ chemical fixation while collecting samples . In the past, the simple and direct sample collection method was to fish biological samples from the deep sea to the deck, and then add chemical reagents for fixation. [0003] The main problems and deficiencies in the prior art include: [0004] Since most deep-sea biological samples have died for a long time when they are caught on the sea s...

AI Technical Summary

Problems solved by technology

[0004] Since most deep-sea biological samples have died for a long time when they are caught on the sea surface, the gene expression in the cells and the three-dimensional structure of chromatin have undergone great changes, resulting in the inability to obtain more scientifically meaningful biological information

In recent years, there have been some technical solutions to release chemical fixatives through the extrusion of deep-sea submersible manipulators. However, the number of deep-sea deep-sea submersibles with a depth of more than 1,000 meters and manipulators is very small, which greatly limits the application of this scheme.

The high-pressure simulation chamber can simulate the high-pressure environment of the deep sea, and is often used to study the adaptability of deep-sea organisms to the high-pressure environment. In order to obtain scientifically meaningful biological information, in-situ chemical fixtures are also required. However, the interior of the high-pressure simulation chamber Limited capacity, chemical fixtures that rely on manipulators or rely on deep submersibles obviously can't be used

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