Particle arrangement method for multiple biochemical detection

Abstract

The invention discloses a particle arrangement method for multiple biochemical detection. According to the method, a micro-fluidic chip and at least N immune particle complexes with different sizes are specifically utilized, and N is an integer greater than 2; and solutions mixed with N immune particle complexes with different sizes are injected into a wedge-shaped inner cavity of a micro-fluidicchip from a fluid inlet of the micro-fluidic chip at a flow speed of 50-300 [mu]L / min, and the N immune particle complexes with different sizes are naturally arranged in the inner cavity of the micro-fluidic chip respectively under the action of a fluid. The method is simple and easy to operate, a detection result is accurate, sensitive and free of optical interference, the chip is not prone to blockage, multiple encoding can be achieved, and the method is widely applied to detection of biochemical markers such as protein, nucleic acid and pathogenic bacteria in the fields of clinical diagnosis, biochemical analysis and drug screening and has wide application prospects.

Description

technical field [0001] The invention relates to a particle array method for multiple biochemical detection. The invention can be widely used in the detection of biochemical markers such as proteins, nucleic acids, and pathogenic bacteria in the fields of clinical diagnosis, biochemical analysis, and drug screening. Background technique [0002] Multiple biochemical detection is of great significance for the rapid and efficient diagnosis of biomedical research and clinical diseases. In biomedical research, the development of many diseases involves the synergistic participation of multiple biochemical detection indicators, so it is necessary to simultaneously perform the detection and content analysis of multiple biochemical markers from one sample. For example, cancer marker screening, eight preoperative items (HBV / HCV / HIV / TP, etc.), and respiratory infection pathogens (influenza, pulmonary bronchial, adenovirus), etc. Limited by the extremely high requirements of multiple ...

AI Technical Summary

Problems solved by technology

The disadvantage of this chip is that the microspheres are fixed on the step boundary, which easily leads to the blockage of the microchannel; especially in multiple biochemical detection, the more types of microspheres are used, the greater the possibility of the fluid channel being blocked, which may cause Affect hybridization efficiency, resulting in reduced sensitivity of detection method

Furthermore, in optical detection, the structural change of the step chip at the step will lead to optical interference such as refraction and shadows in the process of optical detection, which will cause deviations in the accurate calculation of light intensity in the later detection process

Finally, the detection of the target object by microspheres in the microfluidic chip needs to ensure sufficient contact between the two. Therefore, the multiple biochemical detection method of immobilizing the microspheres first and then performing hybridization may easily lead to problems such as low detection efficiency and false positives.

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