Nucleic acid detection chip device, nucleic acid detection chip and preparation method of nucleic acid detection chip

Abstract

The invention relates to the technical field of nucleic acid detection, and discloses a preparation method of a nucleic acid detection chip. The preparation method comprises the following steps: preparing a modification chip, a reaction chip and a valve chip, sealing the reaction chip and the valve chip, and sealing the reaction chip and the valve chip with a modified bottom plate to form the nucleic acid detection chip. Structures of the nucleic acid detection chip and a nucleic acid detection chip device are also disclosed. The nucleic acid detection chip can realize high-sensitivity, low-cost, easy-to-operate and high-accuracy nucleic acid detection and realize detection of coronaviruses such as SARS and various pathogenic nucleic acids such as influenza viruses.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid detection chip device, a nucleic acid detection chip and a preparation method thereof. Background technique [0002] According to the recommendations of the "Technical Guidelines for Laboratory Detection of Pneumonia Infected by Novel Coronavirus" (Sixth Edition), the current routine detection method for the new coronavirus (SARS-CoV-2) is RT-qPCR technology. The application of RT-qPCR technology first requires the purification of viral nucleic acid, and the testing personnel need to directly contact the exposed high-concentration virus particles and genomic nucleic acid for a long time, which has a high risk of infection and requires a laboratory environment with a biosafety level of 2 or above , thus limiting the speed of patient detection, and unable to meet the detection needs of emerging infectious disease pathogens. On the other hand, the puri...

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Problems solved by technology

[0009] Since it takes time for immunization to produce antibodies, it is impossible to detect the inevitable defects of virus antibody detection in the first place

[0010] Because the new coronavirus is highly contagious and easily mutated, the current detection of the new coronavirus faces several major problems: the detection needs to take samples in professional places, process samples in a closed environment, need to use qPCR instruments and be operated by professionals, false positives and False negatives are high, detection throughput is low, strain typing cannot be performed at the same time, etc.

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