Responsive nanoparticles with mitochondrion-targeted function, preparation method therefor and application of responsive nanoparticles

A nanoparticle and responsive technology, applied in the field of nanomaterials, can solve the problems of single function of mitochondria and limited damage of mitochondria, and achieve the effects of inhibiting calcium ion excretion, synergistic treatment of mitochondria, high thermal stability and chemical stability

Active Publication Date: 2020-12-29
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing materials that can damage mitochondr...

Method used

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  • Responsive nanoparticles with mitochondrion-targeted function, preparation method therefor and application of responsive nanoparticles
  • Responsive nanoparticles with mitochondrion-targeted function, preparation method therefor and application of responsive nanoparticles
  • Responsive nanoparticles with mitochondrion-targeted function, preparation method therefor and application of responsive nanoparticles

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preparation example Construction

[0035] The invention provides a method for preparing responsive nanoparticles with mitochondrial targeting function, comprising: S1) mixing inorganic calcium salt, dopamine and alcohol solvent to obtain a precursor solution; S2) mixing the precursor solution with carbonic acid Ammonium hydrogen is placed in a closed environment without contact with each other, and heated to react to obtain responsive nanoparticles.

[0036] Among them, the present invention has no special limitation on the types of all raw materials, as long as they are commercially available.

[0037] The inorganic calcium salt, dopamine and alcohol solvent are mixed to obtain a precursor solution; the inorganic calcium salt is preferably calcium chloride and / or calcium nitrate; the alcohol solvent is preferably ethanol; the mass ratio of the inorganic calcium salt to dopamine Preferably (10-20): 1, more preferably (12-18): 1, more preferably (14-16): 1, most preferably 15: 1; the ratio of the inorganic calci...

Embodiment 1

[0053] (1) Weigh 150 mg of calcium chloride, 10 mg of dopamine, and 20 mg of curcumin and add them to a beaker with 100 ml of ethanol for dissolution.

[0054] (2) Weigh 10 mg of cisplatin and dissolve it in 2 ml of DMSO, and disperse the solution into the above (1) beaker A.

[0055] (3) Weigh 5 grams of ammonium bicarbonate and place it in beaker B.

[0056] (4) Place the A and B beakers in a vacuum desiccator at 40° C., and react for 10 h.

[0057] (5) Centrifuge at 8000rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain responsive nanoparticles, which are designated as PDACaPtCur nanoparticles.

[0058] (6) Disperse the PDACaPtCur nanoparticles in (5) in 30 ml of ethanol, add 10 ml of 20 mg / ml PEI ethanol solution (molecular weight of polyethyleneimine 800), and stir overnight.

[0059] (7) Centrifuge at 8000rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain polyethyleneimine-modified responsive nanopar...

Embodiment 2

[0077] (1) Weigh 150 milligrams of calcium chloride and 10 milligrams of dopamine into a beaker containing 100 milliliters of ethanol for dissolution.

[0078] (2) Weigh 5 grams of ammonium bicarbonate and place it in beaker B.

[0079] (3) Place the A and B beakers in a vacuum desiccator at 40° C., and react for 10 h.

[0080] (4) Centrifuge at 8000rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain responsive nanoparticles, which are designated as PDACa nanoparticles.

[0081] (5) Disperse the PDACa nanoparticles in (4) in 30 ml of ethanol, add 10 ml of 20 mg / ml PEI ethanol solution (molecular weight of polyethyleneimine is 800), and stir overnight.

[0082] (6) Centrifuge at 8000rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain polyethyleneimine-modified responsive nanoparticles, which are designated as PEICa nanoparticles.

[0083] (7) Disperse the PEICa nanoparticles in (6) in 30 ml of ethanol, add 10...

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Abstract

The invention provides a preparation method for responsive nanoparticles with a mitochondrion-targeted function. The method comprises the steps: S1) mixing an inorganic calcium salt, dopamine and an alcohol solvent, so as to obtain a precursor solution; and S2) placing the precursor solution and ammonium bicarbonate in a closed environment in a noncontact manner, and carrying out heating for a reaction, so as to obtain the responsive nanoparticles. Compared with the prior art, the responsive nanoparticles prepared by the method has the advantages that the responsive nanoparticles are uniform in shape and uniform in size, have relatively high thermal stability and chemical stability, can achieve excited response of a variety of light of different wavelengths, can continuously generate calcium ions in tumor cells to increase a concentration of the calcium ions in the cells, and can also cooperate with micromolecular drugs to inhibit a calcium ion discharging action of a mitochondrion andcooperate with the micromolecular drugs to jointly destroy functions of the mitochondrion so as to promote damage of the mitochondrion, and thus, the aim of tumor therapy is achieved, so that the responsive nanoparticles have great potentials in cancer therapy in the future.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a responsive nanoparticle with mitochondrial targeting function, its preparation method and application. Background technique [0002] In order to effectively treat cancer, most drugs need to act on specific organelles, so there are more and more researches on organelle-targeted drug delivery systems in recent years. [0003] Mitochondria are one of the important components in cells and have multiple functions, such as regulating apoptosis, energy conversion, tricarboxylic acid cycle, oxidative stress, storing calcium ions, etc., among which storing calcium ions is one of its important functions . More than 90% of intracellular calcium ions are stored in the intracellular calcium pools - endoplasmic reticulum and mitochondria. Mitochondrial uptake of Ca 2+ It relies on the calcium transporter on the mitochondrial membrane, which is a protein that exists on th...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/02A61K47/34A61K47/10A61K33/243A61K31/12A61P35/00
CPCA61K9/5115A61K9/5146A61K33/243A61K31/12A61P35/00A61K2300/00
Inventor 丁建勋郑盼丁彬彬李杲陈学思
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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