Cultivation method of disease-resistant homozygote litchi trees
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A breeding method and homozygous technology, applied in the field of crop breeding, can solve the problems of long breeding cycle and low breeding success rate, and achieve the effects of good quality, good disease resistance and improved disease resistance
Inactive Publication Date: 2021-01-05
海南瑞民农业科技有限公司
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Problems solved by technology
[0004] In traditional breeding, the acquisition of disease-resistant high-generation pure and inbred parent materials mainly uses self-crossing, hybridization and backcrossing techniques, and the breeding cycle is long and the breeding success rate is low.
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Embodiment 1
[0023] A method for cultivating a disease-resistant homozygous litchi tree, comprising the following steps:
[0024] a, selection and pretreatment of explants: select flower buds whose petals have opened but have not exposed anthers, put them into sterilized nutrient solution after disinfection, and soak for 5 days under dark conditions; wherein, the composition of nutrient solution is as follows: Mass concentration meter, hazelnut extract 2g / L, IAA 0.02mg / L, K 3 PO 4 270mg / L, Na 3 PO 4 340mg / L, sucrose 10g / L;
[0025] b. Haploid plant culture: insert the anthers into the differentiation medium aseptically, and cultivate them under the conditions of light intensity of 1600 lux, light time of 6 hours per day, and temperature of 25°C until the buds differentiated from the anthers grow to 3 cm. , cutting adventitious buds, and inserting them into the rooting medium for cultivation, after cultivating for 42 days under the conditions of light intensity of 1800 lux, light time ...
Embodiment 2
[0031] The difference between embodiment 2 and embodiment 1 is:
[0032] The hazelnut extract is obtained by crushing the hazelnuts, adding water twice the weight of the hazelnuts, ultrasonically extracting at 30° C. for 40 minutes with an extraction power of 300 kW, filtering, and concentrating the filtrate to 70% of the original volume to obtain the hazelnut extract.
Embodiment 3
[0034] The difference between embodiment 3 and embodiment 1 is:
[0035] The blackcurrant extract is obtained by washing the blackcurrant fruit and crushing it, adding water 30 times the weight of the blackcurrant, extracting at 80° C. for 3 hours, filtering, and concentrating the filtrate to 70% of the original volume to obtain the blackcurrant extract.
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Abstract
The invention provides a cultivation method of disease-resistant homozygote litchi trees. According to the cultivation method, after flower buds are treated with a nutrient solution, complete haploidplants are cultured through anther, the haploid plants are inoculated into a peronophythora litchii culture medium, and disease-resistant plants are screened out; and colchicine is adopted to treat the disease-resistant plants to obtain homozygote plants, then the homozygote plants are subjected to selfing, and the homozygote plants which are consistent in character, excellent in quality and goodin disease resistance are screened out. According to the cultivation method, a tissue culture technology is adopted to culture the haploid plants with peronophythora litchii resistance, homozygote plants are obtained through chromosome doubling, finally the homozygote plants are subjected to selfing to obtain the homozygote plants with peronophythora litchii resistance, consistent characters, excellent quality and good disease resistance, so that the time for obtaining the disease-resistant homozygote plants by traditional breeding is shortened, and the process is simple and easy to popularize.
Description
technical field [0001] The invention relates to the field of crop breeding, in particular to a method for cultivating disease-resistant homozygous litchi trees. Background technique [0002] Litchi is an evergreen tree belonging to Sapindaceae. Flowering in spring, fruiting in summer. The pulp is translucent and creamy when it is fresh, and has a delicious taste, but it is not resistant to storage. Lychee is sweet, sour, warm in nature, enters the heart, spleen, and liver meridian; it can stop hiccups and diarrhea, and is a good food therapy for intractable hiccups and early diarrhea. At the same time, it can nourish the brain and strengthen the spleen. The effect. Because of the hot nature, eating too much is easy to get angry. Litchi wood is solid, elegant in texture, and resistant to decay. It has always been a top-grade famous material. The trunk of lychee is more delicate and smooth. Generally, the trunk may be a little light green, and the branches may have some s...
Claims
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