Molecular markers for screening and diagnosing early stage of cervical lesion and application of molecular markers

Abstract

The invention belongs to the technical field of molecular biomedicine, and particularly relates to a high-mobility protein HMGB3 and a gene thereof as molecular markers for screening and diagnosing early stage of cervical lesion and application of the molecular markers. According to the molecular markers and the application thereof, the differential genes in each progress stage of the cervical lesion are screened out from a GEO database, then statistics is carried out according to the expression conditions of the differential genes in the lesion tissue, so that the gene HMGB3 with higher priority is screened out for subsequent verification. Verification experiments prove that the expression positive rate of HMGB3 is far higher than the positive rate of an existing diagnosis method in the cervical epithelium CIN1 level, namely cervical primary lesion, it is indicated that the sensitivity of HMGB3 serving as the marker has obvious advantages compared with the existing diagnosis method, and HMGB3 can be used for early detection, screening and diagnosis of cervical lesion.

Description

technical field [0001] The invention belongs to the technical field of molecular biomedicine, and specifically relates to high-mobility protein HMGB3 as a molecular marker for screening and diagnosis of cervical lesions in the early stage and the application of the molecular marker. [0002] technical background [0003] Cervical cancer is the second most common cancer in women next to breast cancer. According to statistics, about 200,000 women die of cervical cancer every year around the world. And in recent years, the age of onset of cervical cancer tends to be younger (Haimiti A, Hailiman Y, Gulina A, et al. Reduced expression of members of the mhc-i antigen processing machinery in ethnic Uighur women with cervical cancer in the Xinjiang region of China .Curr Oncol 2014; 21:67–74), cervical cancer is not easy to detect in the early stage, and often has lesions or even developed into cancer when it is discovered, so its early screening is particularly important. [0004] T...

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Problems solved by technology

Therefore, p16 staining has limited value indicating the outcome of histological low-grade squamous intraepitheliallesions of the cervix.Mod Pathol.2016; 29(1):51-59.doi:10.1038 / modpathol.2015.126), and the staining of Ki67 in tissues is also variable, and the specificity is not high (SariAslani F, Safaei A, Pourjabali M, et al. Evaluation of Ki67,p16 and CK17 markers in differentiating cervical intraepithelial neoplasia and benign lesions. IranJ Med Sci 2013; 38:15–21.)

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