Molecular markers for screening and diagnosing early stage of cervical lesion and application of molecular markers

A technique for molecular markers and cervical lesions, which is applied in analytical materials, biological tests, biochemical equipment and methods, and can solve the problems of low marker value and low specificity

Active Publication Date: 2021-01-05
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, p16 staining has limited value indicating the outcome of histological low-grade squamous intraepitheliallesions of the cervix.Mod Pathol.2016; 29(1):51-59.doi:10.1038/modpathol.2015.126), and the staining of Ki67 in tissues is

Method used

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  • Molecular markers for screening and diagnosing early stage of cervical lesion and application of molecular markers
  • Molecular markers for screening and diagnosing early stage of cervical lesion and application of molecular markers
  • Molecular markers for screening and diagnosing early stage of cervical lesion and application of molecular markers

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Embodiment 1

[0021] Example 1: Data screening and integration analysis

[0022] Experimental method: The GEO database is a public repository of functional genomic data developed by NCBI (National Center for Biotechnology Information, USA). Two gene expression profiles were retrieved in the GEO database: GSE7803 (from the platform GPL96[HG-U133A]Affymetrix Human Genome U133A Array, containing 10 samples of normal cervical squamous epithelium, 7 samples of HSIL and 21 samples of CSCC); GSE51993 (from platform Illumina Human v2 MicroRNAexpression beadchip and platform Illumina HumanHT-12V4.0 expression beadchip, each platform contains 9 CIN1 cervical lesion samples, 8 CIN3 cervical lesion samples and 7 normal cervical squamous epithelial samples).

[0023] GEO2R is a network analysis tool used to compare two or more samples in a GEO dataset to identify differential genes in different samples. The GEO2R tool was used to analyze the differential genes between the HSIL, CSCC group and normal ce...

Embodiment 2

[0031] Example 2. Statistics on protein expression of co-expressed differential genes in tissues

[0032] The Human Protein Atlas (HPA) online database provides information on the distribution of 26,000 human proteins in tissues and cells, and detects the expression of each protein in cell lines, normal tissues and tumor tissues by Western blot, immunofluorescence, and immunohistochemical techniques Condition. The present invention makes statistics on the protein expression of 48 common differential genes in normal cervical tissue and cervical cancer tissue, and screens out potential markers of cervical lesions according to the standard of negative expression in normal cervical epithelial tissue and positive expression in cervical cancer tissue .

[0033] In order to verify the expression of 34 up-regulated co-expression differential genes and 14 down-regulation co-expression differential genes in different cervical tissues, the 34 common up-regulation genes and 14 common up-...

Embodiment 3

[0035] Example 3. Tissue sample screening and immunohistochemical verification

[0036] 1. HE staining and immunohistochemistry of tissue

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Abstract

The invention belongs to the technical field of molecular biomedicine, and particularly relates to a high-mobility protein HMGB3 and a gene thereof as molecular markers for screening and diagnosing early stage of cervical lesion and application of the molecular markers. According to the molecular markers and the application thereof, the differential genes in each progress stage of the cervical lesion are screened out from a GEO database, then statistics is carried out according to the expression conditions of the differential genes in the lesion tissue, so that the gene HMGB3 with higher priority is screened out for subsequent verification. Verification experiments prove that the expression positive rate of HMGB3 is far higher than the positive rate of an existing diagnosis method in the cervical epithelium CIN1 level, namely cervical primary lesion, it is indicated that the sensitivity of HMGB3 serving as the marker has obvious advantages compared with the existing diagnosis method, and HMGB3 can be used for early detection, screening and diagnosis of cervical lesion.

Description

technical field [0001] The invention belongs to the technical field of molecular biomedicine, and specifically relates to high-mobility protein HMGB3 as a molecular marker for screening and diagnosis of cervical lesions in the early stage and the application of the molecular marker. [0002] technical background [0003] Cervical cancer is the second most common cancer in women next to breast cancer. According to statistics, about 200,000 women die of cervical cancer every year around the world. And in recent years, the age of onset of cervical cancer tends to be younger (Haimiti A, Hailiman Y, Gulina A, et al. Reduced expression of members of the mhc-i antigen processing machinery in ethnic Uighur women with cervical cancer in the Xinjiang region of China .Curr Oncol 2014; 21:67–74), cervical cancer is not easy to detect in the early stage, and often has lesions or even developed into cancer when it is discovered, so its early screening is particularly important. [0004] T...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574G01N33/68
CPCC12Q1/6886G01N33/57484G01N33/57411G01N33/6893C12Q2600/112C12Q2600/158
Inventor 杨武林崔倩文刘雨赵加荣王宏志戴海明
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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