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Use of gmfb as a biomarker for hepatocellular carcinoma

A hepatocellular carcinoma and biomarker technology, applied in the application field of GMFB as a hepatocellular carcinoma biomarker, can solve the problems of insufficient AFP sensitivity, unsatisfactory specificity and sensitivity, and unsatisfactory detection specificity of AFP

Active Publication Date: 2022-04-19
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specificity and sensitivity of AFP to HCC are not ideal
About 40% of HCC patients have normal AFP levels, especially for early HCC, the sensitivity of AFP detection is insufficient
It is also found clinically that the AFP level is also elevated in some patients with chronic hepatitis and liver cirrhosis, which shows that the specificity of AFP detection is not ideal.
There is no research on the relationship between GMFB and hepatocellular carcinoma

Method used

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  • Use of gmfb as a biomarker for hepatocellular carcinoma
  • Use of gmfb as a biomarker for hepatocellular carcinoma
  • Use of gmfb as a biomarker for hepatocellular carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Using the UALCAN website (http: / / ualcan.path.uab.edu / index.html), GMFB TPM was performed on the RNA-seq data of 50 normal people and 371 HCC tissues in the TCGA database ( Transcripts Per Million) analysis [see literature 1,2,3].

[0041] The results showed that GMFB was highly expressed in HCC tissues. The median GMFB TPM of the normal population was 6.04, the upper quartile was 8.012, and the lower quartile was 5.021. The median of the GMFB TPM group in HCC patients was 12.07, the upper quartile was 17.524, and the lower quartile was 8.123. The Student's t test showed that the difference was statistically significant (P=1.62436730732907E-12), so the RNA expression level of GMFB can be used as a marker molecule for the diagnosis of hepatocellular carcinoma. Once the TPM of GMFB in liver tissue is higher than 12.07, it means that the patient is extremely possible hepatocellular carcinoma.

[0042] figure 1 It shows that GMFB is highly expressed in various cancers in...

Embodiment 2

[0044] Kaplan-Meier Plotter (https: / / kmplot.com / analysis / ) was used to analyze the prognosis of 364 HCC patients with different expression levels of GMFB in the TCGA database [see Document 4].

[0045] The expression level of GMFB was significantly correlated with the prognosis of HCC patients. The expression range of the probes in the data was 434-5082, and the CUTOFF value used in the analysis was 1481. The median survival period of patients with low expression was 82.9 months, and that of patients with high expression was 46.2 months, the difference was statistically significant (P=0.0042). Then, 246 male patients were analyzed. It was found that the expression of GMFB had a more significant correlation with the prognosis of male patients. The expression range of the probes in the data was 434-5082, and the CUTOFF value used in the analysis was 1958. The median survival period of patients with low expression was 84.7 months, and that of patients with high expression was ...

Embodiment 3

[0049] Using the UALCAN website (http: / / ualcan.path.uab.edu / index.html), the TPM of GMFB in different grades and stages of HCC in the TCGA database was analyzed.

[0050] During the staging analysis of hepatocellular carcinoma, 50 samples from normal people, 168 samples from stage 1, 84 samples from stage 2, 82 samples from stage 3 and 6 samples from stage 4 were randomly selected, and their GMFB TPM The digits are 6.04, 11.056, 11.233, 12.515 and 12.812 respectively. In the process of grading analysis of hepatocellular carcinoma, 50 samples of normal population, 54 samples of grade 1, 173 samples of grade 2, 118 samples of grade 3 and 12 samples of grade 4 were randomly selected. GMFB TPM The medians are 6.04, 10.824, 11.684, 13.588 and 9.358, respectively. Among them, stage 4 and grade 4 were not statistically different because the patients entered the terminal stage and the number of cases was small.

[0051] Figure 4 and Figure 5 It shows that the expression level of...

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Abstract

The invention provides the application of GMFB as a biomarker of hepatocellular carcinoma, belonging to the technical field of cancer diagnosis and treatment. The present invention takes a large amount of sequencing data and clinical data in the TCGA database as the research object, statistically analyzes the expression level of GMFB in the population of patients with hepatocellular carcinoma, and shows that GMFB is a significantly differentially expressed gene of hepatocellular carcinoma HCC, and GMFB is related to the pathogenesis of HCC. GMFB is related to the pathogenesis of HCC, and has a strong correlation with the grade and stage of HCC, and is also significantly related to the prognosis of HCC patients, especially male patients. Application of the reagent for detecting the expression level of GMFB in the preparation of kits for early diagnosis of hepatocellular carcinoma, grading and / or staging of hepatocellular carcinoma or assessment of prognosis of hepatocellular carcinoma. Inhibiting the expression of GMFB can inhibit the metastasis of hepatocellular carcinoma cells, so the application of GMFB in the preparation of drugs for preventing and / or treating hepatocellular carcinoma or hepatocellular carcinoma.

Description

technical field [0001] The invention belongs to the technical field of cancer diagnosis and treatment, and in particular relates to the application of GMFB as a biomarker for hepatocellular carcinoma. Background technique [0002] Primary liver cancer is one of the most common malignant tumors in the world, characterized by rapid growth and strong invasiveness. Among them, hepatocellular carcinoma (Liver hepatocellular carcinoma, HCC, LIHC) accounts for 75% to 90% of primary hepatocellular carcinoma. HCC is often secondary to chronic diseases, including chronic liver cell lesions such as hepatitis B, and systemic diseases such as obesity and metabolic syndrome. The high morbidity and mortality of HCC have brought huge financial and social burdens to our country. [0003] Due to the lack of sensitive early detection methods, HCC is usually associated with a poor prognosis, with a 5-year survival rate of less than 5%. At present, according to the monitoring guidelines for e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61P35/00
CPCC12Q1/6886G01N33/57438G01N33/57484A61K45/00A61P35/00C12Q2600/112C12Q2600/158C12Q2600/118G01N2800/56G01N2800/52
Inventor 吕立夏徐国彤孙婉张介平王娟高芙蓉金彩霞田海滨徐晶莹欧庆键
Owner TONGJI UNIV