A kind of c-reactive protein and serum amyloid A combined detection test strip, kit and preparation method of test strip

Abstract

The present application discloses a test strip for joint detection of C-reactive protein and serum amyloid A, a kit and a preparation method for the test strip. The test strip comprises a bottom plate, a sample pad, a binding pad, a reaction pad and a water-absorbing pad; the binding pad is coated with fluorescent microspheres jointly labeled with CRP antibody and CRP broadened rabbit anti-antibody, SAA antibody and SAA broadened chicken anti-antibody joint label Fluorescent microspheres; CRP detection line, SAA detection line, CRP widening line and SAA widening line are formed on the reaction pad. The test strip and the test kit containing the test strip of this application can simultaneously quantitatively detect the content of CRP and SAA in the sample, with high sensitivity, good specificity, fast detection speed, and a very wide linear range. The linear range of CRP is 0.5-600mg / L, the linear range of SAA is 3-800mg / L, which reduces the repeated dilution of samples in clinical practice and improves the efficiency of clinical diagnosis.

Description

technical field [0001] The present application relates to the technical field of immunoassay, in particular to a test strip for joint detection of C-reactive protein and serum amyloid A, a kit and a preparation method for the test strip. Background technique [0002] C-reactive protein (CRP) is a part of the body's non-specific immune mechanism. It binds C-polysaccharides, and in the presence of Ca ions, it can bind to phosphorylcholine on the cell membrane, activate the classic pathway of complement, enhance leukocyte phagocytosis, and remove pathogens that invade the body. Microorganisms and damaged, necrotic, apoptotic tissue cells. [0003] Serum amyloid A (SAA) is an acute reaction protein. In a healthy state, its content in plasma is low. When the body is infected by viruses, bacteria, mycoplasma, etc., it can rise rapidly within 4-6 hours. It is about 1000 times higher, but when the stimulating factor is completely eliminated, it will decrease rapidly until it return...

AI Technical Summary

Problems solved by technology

However, the above-mentioned kit only has two detection lines and one quality control line, and the linear range of detection is relatively narrow. For high-value samples, direct detection cannot be carried out. The samples need to be diluted multiple times before loading and testing. The operation steps are cumbersome. greatly limit its application

Therefore, there is still a lack of CRP and SAA combined detection kits with the characteristics of fast, accurate quantification, and high linear range at the same time, which are used for auxiliary diagnosis

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