Method for judging cold and heat properties of vegetable food
A plant-based, food-based technology, applied in the direction of testing food, testing plants/trees, measuring devices, etc., to achieve the effect of small application scope, simple operation process, and uniform application scope
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Embodiment 1
[0029] (1) Preparation of medium for the sample to be tested (white tea): mash and homogenize white tea to extract water-soluble components, and add it to the blank medium at a final mass fraction of 5% (by dry weight).
[0030] (2) The unmated female Drosophila flies that had eclosion within 10 h were selected, and after anesthesia, they were randomly divided into 2 groups, 50 in each group, and raised in blank medium.
[0031] (3) After 3 days of culture in the blank medium, the female fruit flies were respectively transferred into the sample medium to be tested and the blank medium for 3 days, and the fruit flies cultured in the blank medium were used as the blank control group, (feeding conditions: temperature 26 ℃, humidity 50%, light: dark=12h : 12h).
[0032] (4) Transfer the female fruit flies after 3 days of culture into empty bottles, and perform uniform heat exposure in an artificial climate box at (36±1)°C.
[0033] (5) Using heat tolerance indicators (including b...
Embodiment 2
[0038] (1) Preparation of test sample (fructose syrup) medium: Add fructose syrup to the blank medium at a final mass fraction of 5%.
[0039] (2) The unmated female Drosophila flies that had eclosion within 10 h were selected, and after anesthesia, they were randomly divided into 2 groups, 50 in each group, and raised in blank medium.
[0040] (3) After 3 days of culture in the blank medium, the female fruit flies were transferred into the sample medium to be tested and the blank medium for culture for 3 days, and the fruit flies cultured in the blank medium were used as the blank control group. Feeding conditions: temperature 26 ℃ , humidity 50%, light: dark=12h : 12h.
[0041] (4) Transfer the female fruit flies after 3 days of culture into empty bottles, and perform uniform heat exposure in an artificial climate box at (36±1)°C.
[0042] (5) Using heat tolerance indicators (including but not limited to the half-lethal time (LT) of Drosophila 50 ), average survival time (...
Embodiment 3
[0047] (1) Preparation of test sample (glucose) medium: Glucose was added to the blank medium at a final mass fraction of 5%.
[0048] (2) The unmated female Drosophila flies that had eclosion within 10 h were selected, and after anesthesia, they were randomly divided into 2 groups, 50 in each group, and raised in blank medium.
[0049] (3) After 3 days of culture in the blank medium, the female fruit flies were respectively transferred into the sample medium to be tested and the blank medium for 3 days, and the fruit flies cultured in the blank medium were used as the blank control group, (feeding conditions: temperature 26 ℃, humidity 50%, light: dark=12h : 12h).
[0050] (4) Transfer the female fruit flies after 3 days of culture into empty bottles, and perform uniform heat exposure in an artificial climate box at (36±1)°C.
[0051] (5) Using heat tolerance indicators (including but not limited to the half-lethal time (LT) of Drosophila 50 ), average survival time (T), ma...
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